蒲忠慧^1，2，3，李 涛⁴，魏永孝³，邓静竹¹，李 丽¹，熊 亮^2*，李刘英^1*

（1.自贡市第一人民医院，自贡643000；2.成都中医药大学药学院，成都611137；3.成都医学院检验医学院，成都610500；4.成都医学院第二附属医院·核工业四一六医院，成都610051）

摘要：研究生化汤对急性血瘀模型大鼠的活血化瘀作用。将48只SD大鼠随机分为空白组、模型组、阳性组（复方丹参滴丸组，73mg/kg）和生化汤低、中、高剂量组（25、50、100mg/kg），采用皮下注射肾上腺素联合冰水浴制备急性血瘀证大鼠模型，测定各组大鼠的全血黏度（Whole blood viscosity，WBV）、血浆黏度（Plasma viscosity，PV）、红细胞变形指数（Erythrocyte deformation index，EI）、红细胞聚集指数（Erythrocyte aggregation index，EAI）、红细胞压积（Hematocrit，HCT）等血液流变学指标以及凝血酶原时间（Prothrombin time，PT）、活化部分凝血酶原时间（Activated partial prothrombin time，APTT）、凝血酶时间（Thrombin time，TT）、血浆纤维蛋白原（Fibrinogen，FIB）等凝血指标；HE染色观察肺组织病理变化。结果表明，与空白组相比，模型组大鼠在不同切变率（1、50、200s-1）下的WBV及PV、EAI 和HCT 显著或极显著升高（P＜0.05、P＜0.01），EI 极显著降低（P＜0.01），PT、APTT、TT 显著或极显著缩短（P＜0.05、P＜0.01），FIB显著升高（P＜0.05）。与模型组相比，生化汤剂量组大鼠在不同切变率（1、50、200s-1）下的WBV及PV和EAI显著或极显著降低（P＜0.05、P＜0.01），中、高剂量组的HCT极显著降低（P＜0.01），EI极显著升高（P＜0.01），PT和APTT显著或极显著延长（P＜0.05、P＜0.01），中、高剂量组的TT显著或极显著延长（P＜0.05、P＜0.01），高剂量组的FIB显著降低（P＜0.05）。肺组织HE染色结果显示，模型组大鼠肺泡隔增厚、出血、局部肺气肿、红细胞及血红蛋白结晶聚集，生化汤组大鼠肺组织病变程度明显减轻，高剂量组改善更明显。生化汤可通过改善血液流变性、改善止血和凝血病理变化、减轻肺部病理损伤等途径来发挥活血化瘀的作用。

关键词：生化汤；急性血瘀；血液流变学指标；凝血指标

中图分类号：R285.5 文献标识码：A 文章编号：1674-506X（2025）01-0102-0006

Research on the Effects of Shenghua Decoction on Promoting Blood Circulation and Removing Blood Stasis in Acute Blood Stasis Model Rats

PU Zhonghui^1，2，3，LI Tao⁴，WEI Yongxiao³，DENG Jingzhu¹，LI Li¹，XIONG Liang^2*，LI Liuying^1*

（1.Zigong First People Hospital，Zigong 643000，China；2.School of Pharmacy，Chengdu University of Traditional Chinese Medicine，Chengdu 611137，China；3.School of Laboratory Medicine，Chengdu Medical College，Chengdu 610500，China；4.The Second Affiliated Hospital of Chengdu Medical College，Nuclear Industry 416 Hospital，Chengdu 610051，China）

Abstract： To investigate the effects of Shenghua decoction on promoting blood circulation and removing blood stasis in acute blood stasis model rats. 48 SD rats were randomly divided into blank group, model group, positive group (Compound Danshen Dripping Pills group, CDDP, 73 mg/kg), Shenghua decoction low, medium and high dose groups (25, 50, 100 mg/kg). The acute blood stasis model was established by subcutaneous injection of adrenaline and swimming in ice water. Determining hemorheology indexes such as whole blood viscosity (WBV), plasma viscosity (PV), erythrocyte deformation index (EI), erythrocyte aggregation index (EAI), hematocrit (HCT), and blood coagulation indexes such as prothrombin time (PT), activated partial prothrombin time (APTT), thrombin time (TT) and fibrinogen (FIB), histopathological changes of lung tissue were observed by HE staining. Results showed that compared with blank group, in different shear rates (1,50,200 s-1), WBV, PV, EAI and HCT were greatly or significantly increased (P＜0.05, P＜0.01) in model group, EI was decreased significantly (P＜0.01)； PT, TT and APTT were greatly or significantly shortened (P＜0.05, P＜0.01), FIB was prolonged greatly (P＜0.05) . Compared with model group, rats of Shenghua decoction dose groups in different shear rates (1, 50, 200 s-1) decreased greatly or significantly (P＜0.05, P＜0.01) in WBV,PV,EAI. HCT was significantly decreased in medium and high dose groups (P＜0.01), while EI was significantly increased (P＜0.01),and PT,APTT was greatly or significantly prolonged (P＜0.05, P＜0.01) . In medium and high dose groups, TT was greatly or significantly prolonged (P＜0.05, P＜0.01),in high dose group, FIB was shortened greatly (P＜0.05) . Lung tissue HE staining results showed that the alveolar septum of model group rats was thickened, with hemorrhage, localized emphysema, aggregation of red blood cells and hemoglobin crystals. The degree of lung tissue lesions in rats in Shenghua decoction group was obviously reduced, and the effect in high dose group was more obvious. Shenghua decoction may exert the effect of activating blood circulation and removing blood stasis by improving property of hemorheology, improving hemostatic and coagulation pathological changes, and alleviating pathological damage to the lungs.

Keywords：Shenghua decoction；acute blood stasis；hemorheology indexes；blood coagulation indexes

doi：10.3969/j.issn.1674-506X.2025.01-013