赵云龙1，2，李丽鑫1，2，吴 显3，杨磊壮3，杨嘉琪4，庞仕龙1，2，陈 娴1，2，刘 佳3*
（1.昆明市食品药品检验所，云南昆明 650032；
2.昆明市食品药品检验研究院，云南昆明 650032；
3.昆明医科大学海源学院，云南昆明 650101；
4.昆明学院化学化工学院，云南昆明 650032）
摘要：为了建立微波消解-原子荧光光谱法准确快速测定茶叶中硒含量的方法。样品经微波消解后，分别从是否添加铁氰化钾，添加的比例及添加后反应的时间三方面进行考察，确定添加铁氰化钾、试样-铁氰化钾（4∶1，v/v）、添加后反应30 min为实验最佳优化方法。结果表明，硒质量浓度在0～100 ng/mL范围内呈较好线性关系，相关系数R2为0.999 7，检出限为0.011 6 mg/kg，精密度RSD值为0.702%～1.888%，0.015、0.020、0.200 mg/kg三个添加水平下的回收率在86.10%～94.74%之间。该方法简单易操作，灵敏度高、重复性好且稳定，结果准确，适用于茶叶中硒含量的准确快速测定。
关键词：原子荧光光谱；茶叶；硒
中图分类号：TS207.3 文献标识码：A 文章编号：1674-506X（2023）03-0116-0006
Determination of Selenium in Tea by Atomic Fluorescence Spectrometry
ZHAO Yunlong1, 2, LI Lixin1, 2, WU Xian3, YANG Leizhuang3, YANG Jiaqi4, PANG Shilong1, 2, CHEN Xian1, 2, LIU Jia3*
（1.Kunming Institute for Food and Drug Control, Kunming Yunnan 650032, China;
2.Kunming Institute of Food and Drug Inspection, Kunming Yunnan 650032, China;
3.Kunming Medical University Haiyuan College, Kunming Yunnan 650101, China;
4.School of Chemistry and Chemical Engineering, Kunming University, Kunming Yunnan 650032, China）
Abstract：To establish an accurate and rapid method for the determination of selenium content in tea by microwave digestion-atomic fluorescence spectrometry (AFS). After the sample was digested by microwave, it was investigated from whether potassium ferricyanide was added, the ratio of addition and the reaction time after addition. It was sure addition of potassium ferricyanide, sample-potassium ferricyanide (4∶1, v/v) and reaction for 30 min after addition were the best optimization methods for the experiment. The mass concentration of selenium showed a good linear relationship in the range of 0～100 ng/mL, the correlation coefficient R2 was 0.999 7, the detection limit was 0.011 6 mg/kg, and the precision RSD was 0.702%～1.888%, The recoveries at the three addition levels of 0.015, 0.020 and 0.200 mg/kg were between 86.10%～94.74%. The method is simple and easy to operate, with high sensitivity, good repeatability and stability，and accurate results. It is suitable for the accurate and rapid determination of selenium content in tea.
Keywords：atomic fluorescence spectrometer （AFS）; tea; selenium
doi：10.3969/j.issn.1674-506X.2023.03-020
收稿日期：2022-09-28
作者简介：赵云龙（1984-），男，本科，工程师。研究方向：食品质量安全。
*通信作者：刘佳（1983-），女，硕士，副教授。研究方向：药剂学研究。
引用格式：赵云龙,李丽鑫,吴显,等.原子荧光光谱法测定茶叶中的硒含量[J].食品与发酵科技,2023,59(3):116-120,143.
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