郭 燕1，朱凡君2，费建枫3，韦何雯2
（1.浙江药科职业大学，宁波315000；2.金华市食品药品检验检测研究院，金华321000；3.杭州纽太生物科技有限公司，杭州310000）
摘要：建立一种基于上转换发光材料的定量免疫层析检测试纸卡，用于饮用水中苯并［a］芘的快速定量检测，并对所制备的试纸条性能进行评价。采取免疫竞争法，通过优化试纸条各因素，研制苯并［a］芘检测试纸，并对其标准曲线、检测限、定量限、特异性、准确度等方面进行性能评价。结果表明，所制备的试纸卡标准曲线回归方程R2为0.983 5，检测限为1.007 ng/mL，定量限为1.938 ng/mL；与结构类似物无交叉反应；加标回收率为86.00%～108.47%。结果表明所研制的苯并［a］芘免疫层析试纸条具有灵敏度高、特异性好、检测快速、简便的优点，可以用于饮用水中苯［a］并芘的检测。
关键词：苯并［a］芘；免疫层析；上转换发光材料；试纸条
中图分类号：TS207.3 文献标识码：A 文章编号：1674-506X（2024）05-0133-0005
Rapid Detection of Benzo［a］pyrene Residues in Drinking Water by Immunochromatography with Up-conversion Luminescence
GUO Yan1，ZHU Fanjun2，FEI Jianfeng3，WEI Hewen2
（1.Zhejiang Pharmaceutical University，Ningbo 315000，China；2.Jinhua Institute for Food and Drug Control，Jinhua 321000，China；3.Hangzhou New-test Biology Company Limited，Hangzhou 310000，China）
Abstract：A quantitative immunochromatographic test strip was established based on up-conversion luminescence materials for the rapid and quantitative detection of benzo［a］pyrene in drinking water，and the performance of the test strip was evaluated. Based on the competitive immunoassay model， and by optimizing the test strip elements，a benzo［a］pyrene test paper was developed. The performance of the strip was evaluated in terms of its standard curve， detection limit， quantitative limit， specificity， accuracy， etc. The results showed the regression equation R2 value of the standard curve of the developed test strip reached 0.983 5， the detection limit reached 1.007 ng/mL， the quantitative limit reached 1.938 ng/mL. The strip had no cross-reaction with structural analogs of benzo［a］pyrene， the recovery rate was 86.00%～108.47%. The results showed that the developed benzo［a］pyrene immunochromatographic test strip has the advantages of high sensitivity，good specificity， fast and simple detection， and can be applied to the detection of benzo［a］pyrene residues in drinking water.
Keywords：benzo［a］pyrene；immunochromatography；up-conversion luminescence materials；test strip
doi：10.3969/j.issn.1674-506X.2024.05-021
收稿日期：2023-09-04
基金项目：浙江药科职业大学2021年度校级科研项目（2021185）；浙江省基础公益计划项目（LGC22C200008）；浙江省市场监督管理局雏鹰计划培育项目（CY2022355）
作者简介：郭燕（1989-），女，硕士，讲师。研究方向：食品质量与安全。
引用格式：郭燕，朱凡君，费建枫，等.上转换发光免疫层析法快速检测饮用水中苯并［a］芘残留［J］.食品与发酵科技，2024，60（5）：133-136，147.
