杨春梅¹，李兴玉²，陈 涛²，黄 静³，马 倩²，吴雪莉²，李敏婕²，周晓群⁴*，孙 涵¹*

（1.成都市产品质量监督检验研究院，成都610000；2.成都产品质量检验研究院有限责任公司，成都610000；3.四川省食品发酵工业研究设计院有限公司，成都611130；4.四川省产品质量监督检验检测院，成都610100）

摘要：该研究建立了一种超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法（UHPLC-Q-Orbitrap-MS）同时检测预制菜中多种防腐剂和甜味剂的分析方法。样品经乙腈水溶液振荡、离心、净化、过滤后上机，经Waters ACQUITY UPLC HSS T3色谱柱（2.1mm×100mm，1.8μm）分离，以流动相A（甲醇-5mmol/L乙酸铵溶液）和流动相B（乙腈）进行梯度洗脱，在负离子模式下进行检测。结果表明，防腐剂和甜味剂在各自线性浓度范围内，线性关系良好，相关系数为0.9945～1.0000，检出限为0.002～0.10mg/kg，定量限为0.008～0.35mg/kg，低、中、高三个浓度水平加标回收率为70.3%～95.4%，相对标准偏差（RSD）为3.1%～13.8%，该方法可用于预制菜中多种防腐剂和甜味剂的快速检测。

关键词：预制菜；防腐剂；甜味剂；超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法

中图分类号：TS207.5 文献标识码：A 文章编号：1674-506X（2025）04-0117-0007

Simultaneous Determination of Various Preservatives and Sweeteners in Premade Dishes Based on UHPLC-Q-Orbitrap-MS Method

YANG Chunmei¹，LI Xingyu²，CHEN Tao²，HUANG Jing³，MA Qian²，WU Xueli²，LI Minjie²，ZHOU Xiaoqun⁴*，SUN Han¹*

(1.Chengdu Product Quality Supervision and Inspection Institute，Chengdu 610000，China；

2.Chengdu Product Quality Inspection and Research Institute Co.，Ltd.，Chengdu 610000，China；

3.Sichuan Food and Fermentation Industry Research & Design Institute Co.，Ltd.，Chengdu 611130，China；

4.Sichuan Product Quality Supervision，Inspection and Testing Institute，Chengdu 610100，China)

Abstract: This study developed an ultra high performance liquid chromatography- quadrupole/electrostatic field orbital trap high-resolution mass spectrometry （UHPLC-Q-Orbitrap-MS） analysis method for simultaneous detection of multiple preservatives and sweeteners in premade dishes. The sample was subjected to oscillation with acetonitrile aqueous solution，centrifugation，purification，and filtration before being loaded onto the machine，it was separated by Waters ACQUITY UPLC HSS T3 （2.1 mm×100 mm，1.8 μm） chromatographic column，gradient elution was performed using mobile phase A（methanol-5mmol/L ammonium acetate solution） and mobile phase B （acetonitrile），and the detection was performed in negative ion mode. The results showed that preservatives and sweeteners had a good linear relationship within their respective linear concentration ranges， with correlation coefficients ranging from 0.994 5～1.000 0， detection limit ranging from 0.002～0.10 mg/kg， and quantification limit ranging from 0.008～0.35 mg/kg. The spiked recovery rates of low，medium，high three concentration levels ranged from 70.3%～95.4%，and the relative standard deviation （RSD） range was 3.1%～13.8%. This method can be used for rapid detection of multiple preservatives and sweeteners in premade dishes.

Keywords: premade dishes； preservative； sweetener； ultra high performance liquid chromatography-quadrupole/electrostatic field orbital trap high-resolution mass spectrometry

doi: 10.3969/j.issn.1674-506X.2025.04-017