梁 蕾，顾浩峰^*，康永宁，于蓉苗，王佳豪

（安康学院现代农业与生物科技学院，陕西安康 725000）

摘 要：本研究以安康富硒茶为原料，利用单因素实验和正交实验优化茶硒多糖的提取工艺，并通过DPPH·、·OH清除实验和HepG2细胞氧化损伤模型评估茶硒多糖的抗氧化性。结果表明：茶硒多糖的最佳提取工艺：超声预处理（500 W，20 min），液料比30:1 mL/g，提取温度85 ℃，提取时间150 min，茶硒多糖得率为（9.67±1.53）%；茶硒多糖（800 μg/mL）的DPPH·和·OH清除率分别为（74.33±0.32）%和（70.16±0.21）%，均显著高于普通茶多糖；茶硒多糖能够显著缓解H₂O₂诱导的HepG2细胞氧化损伤，且效果强于普通茶多糖。

关键词：富硒茶；硒多糖；正交实验；抗氧化；氧化损伤

中图分类号：TS272      文献标识码：A      文章编号：1674-506X（2023）02-0059-0006

Extraction and Antioxidant Ability of Se-polysaccharidesfrom Se-enriched Tea

LIANG Lei，GU Haofeng^*，KANG Yongning，YU Rongmiao，WANG Jiahao

（College of Modern Agriculture and Biotechnology, Ankang University, Ankang Shaanxi 725000, China）

Abstract: The extraction process of Se-polysaccharides from Se-enriched tea (Se-P-T)was optimized by single factor and orthogonal experiments. The antioxidant ability of Se-P-T was evaluated using DPPH and hydroxyl free radical scavenging experiments and oxidative damage models of HepG2 cells. The results showed that the optimal extraction process of Se-P-T was as follows：ultrasonic pretreatment (500 W, 20 min), liquid-material ratio 30∶1 mL/g, extraction temperature 85 ℃ and extraction time 150 min. Under the optimized condition, the yield of Se-polysaccharides was (9.67±1.53)%. The DPPH and hydroxyl free radical scavenging rate of Se-P-T (800 μg/mL) was (74.33 ± 0.32)% and (70.16 ± 0.21)%, respectively, both of which were higher than polysaccharides from tea(P-T). Se-P-T could significantly alleviate H₂O₂ induced oxidative damage in HepG2 cells, and the effect is more potent than P-T.

Keywords: Se-enriched tea; Se-polysaccharide; orthogonal test; antioxidation; oxidative damage

doi: 10.3969/j.issn.1674-506X.2023.02-009

收稿日期：2023-01-16

基金项目：陕西省教育厅项目（21JK0469）；安康学院高层次人才项目（2021AYQDZR04）；安康学院重点项目（2022AYZD01）；安康学院横向项目（2017AYHX021）

作者简介：梁蕾（1988-），女，硕士，实验师。研究方向：食品科学和功能性食品开发。

*通信作者：顾浩峰（1986-），男，博士，副教授。研究方向：功能性食品和靶向胶体构建。

引用格式：梁蕾,顾浩峰,康永宁,等.安康富硒茶中硒多糖的提取及抗氧化性研究[J].食品与发酵科技,2023,59(2):59-64.