谢煜敏^1，2，李晓敏^1，2，刘洪霞²，詹若挺^1*，丰时运²，谢 果^1，2，3

（1.广州中医药大学中药学院，广东广州 510006；

2.完美（广东）日用品有限公司，广东中山 528400；

3.电子科技大学中山学院，广东中山 528402）

摘要：以人参、黄精、枸杞为主要原料，利用秀丽隐杆线虫对其主要功效成分人参黄精枸杞混合提取液（RHG）的神经保护作用进行研究，并以RHG 为主要原料，添加复合果汁，采用感官评价方式，通过单因素实验和Box-Behnken响应面优化饮料配方。结果表明，不同浓度的RHG均能显著提高线虫ASH神经元存活率，对多聚谷氨酰胺聚集造成的神经毒性均具有一定的抵抗作用；其中中剂量RHG（125 μg/mL）效果最佳，与空白组相比，神经元存活率提升至80.43%，避化率增加至56.94%。人参黄精枸杞复合饮料的最佳配方为RHG添加量39.94%，水蜜桃浓缩汁添加量6.5%，柠檬浓缩汁添加量0.4%，甜菊糖苷添加量0.02%，感官评分平均值为87.0分。人参黄精枸杞复合饮料口感润滑，风味协调，具有一定的神经保护作用，为功能性复合饮料的研制提供依据。

关键词：人参；黄精；枸杞；秀丽隐杆线虫；神经保护活性；响应面优化；复合饮料

中图分类号：TS275.4   文献标识码：A   文章编号：1674-506X（2023）02-0065-0007

Study on the Activity of Ginseng，Polygonati Rhizoma and Wolfberry Extract and Its Beverage Sensory Optimization

XIE Yumin^1，2，LI Xiaomin^1，2，LIU Hongxia²，ZHAN Ruoting^1*，FENG Shiyun²，XIE Guo^1，2，3

（1.College of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou Guangdong 510006, China;

2.Perfect（Guangdong）Co., Ltd., Zhongshan Guangdong 528400, China;

3.Zhongshan College, University Electronic Science Technology of China, Zhongshan Guangdong 528402, China）

Abstract: Ginseng, Polygonati Rhizoma and wolfberry mixed extract（RHG）which consists of ginseng, Polygonati Rhizoma and wolfberry, is the main ingredient of RHG beverage. The neuroprotective effects of RHG on Caenorhabditis elegans were investigated and the beverage formulation was optimized by single-factor experiment and Box-Behnken response surface methodology based on sensory evaluation. The results showed that RHG at different level significantly improved the survival rate of Caenorhabditis elegans ASH neurons and alleviated the neurotoxicity caused by polyglutamine aggregation. The medium dose of RHG（125 μg/mL）showed the best neuroprotective effect with 80.43% increasing in neuron survival rate and 56.94% increasing in avoidance rate compared with the blank group. The best formulation for each ingredient of ginseng, Polygonati Rhizoma and wolfberry compound beverage was 39.94% RHG, 6.5% peach juice concentrate, 0.4% lemon juice concentrate and 0.02% stevioside with a mean sensory score of 87.0 points. The ginseng, Polygonati Rhizoma and wolfberry compound beverage has a lubricious taste, coordinated flavor and certain neuroprotective effect, which provides a basis for the development of functional compound beverage.

Keywords: ginseng; Polygonati Rhizoma; wolfberry; Caenorhabditis elegans; neuroprotective activity; response surface optimization; compound beverage

doi: 10.3969/j.issn.1674-506X.2023.02-010

收稿日期：2022-12-12

基金项目：国家自然科学基金（No.32101903）

作者简介：谢煜敏（1999-），女，硕士研究生。研究方向：天然产物新产品的开发及活性评价。

*通信作者：詹若挺（1970-），男，研究员。研究方向：中药资源可持续利用研究和开发。

引用格式：谢煜敏,李晓敏,刘洪霞,等.人参黄精枸杞提取液活性研究及其饮料感官优化[J].食品与发酵科技,2023,59(2):65-71.