陈建平1，何颂捷1*，谢皓玥1，胡 凤1，谭文渊2
（1.自贡检验检测院，四川自贡 643000；2.四川轻化工大学，四川自贡 643000）
摘要：建立一种保健食品中黄芪甲苷、毛蕊异黄酮葡萄糖苷、甜菜碱、补骨脂素、异补骨脂素和淫羊藿苷的检测方法。样品经甲醇40 ℃超声提取60 min，采用水（A）和0.1%甲酸乙腈（B）作为流动相，利用超高效液相色谱-三重四极杆质谱联用仪，选用电喷雾离子（electron spray ionization，ESI＋）源、正离子检测模式（positive mode，MRM）进行测定。结果表明，6种功能成分的回收率为85.7%～108.5%，该方法实验室内变异系数为0.91%～4.32%（n＝7），方法检出限为0.02～0.60 μg/kg。该方法准确、灵敏、高效，适合于中药类保健食品中特征成分黄芪甲苷、毛蕊异黄酮葡萄糖苷、甜菜碱、补骨脂素、异补骨脂素和淫羊藿苷的分析检测。
关键词：超高效液相色谱-质谱法；正离子模式；保健食品；功能成分；含量测定
中图分类号：TS207.3 文献标识码：A 文章编号：1674-506X（2023）02-0124-0007
Determination of Six Functional Components in Health Food by Ultra-high Performance Liquid Chromatography-triple Quadrupole Mass Spectrometry
CHEN Jianping1，HE Songjie1*，XIE Haoyue1，HU Feng1，TAN Wenyuan2
（1.Zigong Inspection and Testing Institute, Zigong Sichuan 643000, China;
2.Sichuan University of Science and Engineering, Zigong Sichuan 643000, China）
Abstract: To establish a method for the detection of astragaloside IV, calycosin-7-O-β-D-glucoside, betaine, psoralen, isopsoralen and icariin in health food. The samples were ultrasonically extracted with methanol at 40 ℃ for 60 min, water(A) and 0.1% formic acid acetonitrile(B) were used as mobile phases. The samples were determined on ultra performance liquid chromatography triple quadrupole mass spectrometer by electron spray ionization(ESI＋)source and positive ion detection mode (positive mode，MRM). The results showed that the recoveries of the six functional components were 85.7%～108.5%, the intra-laboratory coefficients of variation were 0.91% ～4.32% (n＝7), and the detection limits were 0.02～0.60 μg/kg, respectively. The method is accurate, sensitive and efficient, and it is suitable for the analysis and detection of the characteristic components astragaloside IV, calycosin-7-O-β-D-glucoside, betaine, psoralen, isopsoralen and icariin in traditional Chinese medicine health food.
Keywords：ultra performance liquid chromatography-tandem mass spectrometry; positive mode; functional food; functional components; content determination
doi：10.3969/j.issn.1674-506X.2023.02-019
收稿日期：2022-05-30
基金项目：四川省市场监管系统科技计划项目（SCSJ2021023）
作者简介：陈建平（1986-），男，本科，主管中药师。研究方向：食品药品安全检测。
*通信作者：何颂捷（1993-），男，硕士。研究方向：食品安全检测。
引用格式：陈建平,何颂捷,谢皓玥,等.超高效液相色谱-三重四级杆质谱法测定保健食品中六种功能成分[J].食品与发酵科技,2023,59(2):124-130.
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