许明君，高如意，邓辰辰，柏伟荣，吴 云
（江苏康缘药业股份有限公司，江苏 连云港 222001）
摘要：采用高效液相色谱仪建立测定复方中药保健酒中松果菊苷与毛蕊花糖苷含量的方法。AlltimaC18色谱柱（4.6 mm×250 mm，5 μm），流动相：乙腈-0.1%甲酸水溶液梯度洗脱（流动相A为乙腈，B为0.1%甲酸水，梯度条件为0-30 min，15-27%A，85-73%B；30-40 min，27%A，73%B）；流速：1.0 mL/min；检测波长：330 nm；柱温30 ℃，进样量10 μL。松果菊苷、毛蕊花糖苷分别在0.05-1.60 mg/mL（R2＝0.999 9；n＝6）、0.25-0.80 mg/mL（R2＝1；n＝6）范围内线性关系良好，加样回收率分别为99.52%及97.20%。该方法重复性好，准确，可用于复方中药保健酒中松果菊苷、毛蕊花糖苷含量测定。
关键词：复方中药保健酒；松果菊苷；毛蕊花糖苷；高效液相色谱仪
中图分类号：TS207.3 文献标识码：A 文章编号：1674-506X（2020）01-0100-0006
Simultaneous Determination of Echinacoside and Acteoside in Cistanche Wine by HPLC
XU Ming-jun，GAO Ru-yi，DENG Chen-chen，BAI Wei-rong，WU Yun
（Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang Jiangsu 222001）
Abstract：To establish a method for determination echinacoside and acteoside in healthby wine by HPLC. Alltima C18 column (4.6 mm×250 mm, 5 μm) was used with the mobilephase of acetonitrile -0.1% formic acid at a flow rate of 1.0 mL/min (The mobile phase A was acetonitrile, B was 0.1% formic acid water, the gradient was 0-30 min, 15-27%A, 85-73%B; 30-40 min, 27%A, 73%B). The detection wavelength was set at 330 nm. The column temperature was maintained at 30 ℃. The linear ranges of echinacoside and acteoside were 0.05-1.60 mg/mL (R2＝0.9999; n＝6) and 0.25- 0.80 mg/mL (R2＝1; n＝6), respectiv ely. The recoveries were 99.52% and 97.20% of respectively. The method was reproducible and accurate. It can be used for the quality control of healthby wine.
Keywords：healthby wine; echinacoside; acteoside; HPLC
doi：10.3969/j.issn.1674-506X.2020.01-019
收稿日期：2019-09-10
基金项目：国家重点研发计划（2017YFC1702403）
作者简介：许明君（1987-），男，工程师，硕士，主要从事中药保健品研发。
引文格式：许明君,高如意,邓辰辰,等.HPLC法同时测定复方中药保健酒中松果菊苷和毛蕊花糖苷的含量[J].食品与发酵科技,2020,56(1):100-105.
