龚 洋，雷正达*，李 莉，何 瑞，李进春
（四川省粮油科研所，四川成都 611130）
摘要：建立了富硒稻谷中硒代胱氨酸（SeCys）、亚硒酸根（Se（IV））、硒代蛋氨酸（SeMet）、硒酸根（Se（VI））4种硒形态的高效液相色谱-氢化物发生原子荧光光谱分析方法。稻谷样品采用链霉蛋白酶E酶解提取，40 mmol/L磷酸氢二铵为流动相，经汉密尔顿阴离子交换柱分离后的溶液引入仪器进行氢化物发生-原子荧光光谱法测定。结果表明，4种硒形态标准曲线线性关系良好（R2≥0.999 0），方法检出限为0.008 01-0.059 25 mg/kg，回收率为85.9-103.9%，RSD＜3%。本方法前处理简单，灵敏度高，能有效地分离并检测出稻谷中多种硒形态含量。
关键词：液相色谱；氢化物发生；原子荧光；富硒稻谷；硒形态
中图分类号：TS207.3 文献标识码：A 文章编号：1674-506X（2020）02-0105-0004
HPLC-HG-AFS Determination of Selenium Morphology in Se-rich Paddy
GONG Yang, LEI Zheng-da*, LI Li, HE Rui, LI Jin-chun
（Sichuan Grain and Oil Research Institute, Chengdu Sichuan 611130, China）
Abstract：A method has been developed for the simultaneous determination of 4 selenium species selenocystine (SeCys), Se (IV), selenomethionine (SeMet), and Se (VI) in Se-enriched paddy by liquid chromatography-hydride generation atomic fluorescence spectrometry（HPLC-HG-AFS）. The protease Ehydrolysis was taken as the sample pretreatment method. The mobile phase was 40 mmol/L (NH4)H2PO4. The target compounds were separated on a Hamilton reversed-phase anion exchange column and analyzed by HG-AFS. As shown by the results, good linearity was obtained for all the selenium species, with the linear correlation coefficients higher than 0.999 0. The LODs of the four species were between 0.008 01-0.059 25 mg/kg. Recoveries for the four analytes were in the range of 85.9-103.9%, with the RSD lower than 3%. The method is simple and sensitive. It could effectively separate the four selenium compounds.
Keywords：liquid chromatography; hydride generation; atomic fluorescence; Se-enriched paddy; selenium species
doi：10.3969/j.issn.1674-506X.2020.02-020
收稿日期：2019-12-03
基金项目：四川省2019年度应用基础研究项目（2019YJ0623）
作者简介：龚洋（1990-），女，硕士研究生。研究方向：食品工程。
*通讯作者
引文格式：龚洋,雷正达,李莉,等.液相色谱——氢化物发生原子荧光光谱法测定富硒稻谷中硒的形态[J].食品与发酵科技,2020,56(2):105-108.
