钱娟娟，王克芬，刘胜利，王 帅，王兴吉，刘文龙^*

（山东隆科特酶制剂有限公司，山东省酶制剂生物发酵技术重点实验室，山东临沂 276400）

摘要：对实验室保藏菌种短小芽孢杆菌WS-135进行紫外诱变，筛选出产酶高的菌株A-5，酶活力为22.1 U·mL^-1，较未诱变菌株提高2.37倍。以紫外诱变筛选菌株为出发菌株，进行硫酸二乙酯诱变，筛选得到一株酶活为28.9U·mL^-1的菌株B-7，较未诱变菌株提高1.3倍。B-7菌株连续传代8次，酶活力保持稳定。研究其酶学特性，结果表明温度为35 ℃时酶的活性最好，在pH为7-10时，有较好的酶活性；在40 ℃，pH 9.0、10.0条件下酶活稳定，在常温下保藏8个月后，仍有80%的酶活力，表明该酶稳定性较好。

关键词：碱性纤维素酶；诱变；酶学性质；稳定性

中图分类号：TS201.3   文献标识码：A   文章编号：1674-506X（2020）05-0060-0005

Screening of Alkaline Cellulase Producing Strain WS-135 and Study on Its Enzymatic Characterization

QIAN Juan-juan, WANG Ke-fen, LIU Sheng-li, WANG Shuai, WANG Xing-ji, LIU Wen-long^*

（1.Shandong Longkete Enzyme Co., Ltd., Linyi Shandong 276400, China;

2.Shandong Province Key Laboratory of Enzyme Preparation Fermentation Technology, Linyi Shandong 276400, China）

Abstract：The original strain Bacillus pumilus WS-135 was mutated by UV in the paper, screening of high enzyme producing strain A-5, and its enzyme productivity was 22.1 U·mL^{- 1} and 2.37 fold higher than that of parent strain. The mutant A-5 was mutated by DES, and a strain B-7 with 28.9 U·mL^{- 1} enzyme activity was obtained which was 1.3 fold higher than that of parent strain. The activity of alkaline cellulase remained stable after 8 passages. The optimal temperature and pH of alkaline cellulase were at 35 ℃, pH 7.0-10.0. The activity of alkaline cellulase was stable at 40 ℃, pH 9.0 and pH 10.0. Store for 8 months at 25 ℃, and enzyme activity of alkaline cellulase reduced 20% when compared with the initial enzyme activity.

Keywords：alkaline cellulase; mutagenesis; enzymatic characteristics; stability

doi：10.3969/j.issn.1674-506X.2020.05-010

收稿日期：2019-11-26

基金项目：山东省技术创新项目（201830515049）

作者简介：钱娟娟（1983-），女，硕士，工程师。研究方向：酶制剂研究与开发。

*通讯作者

引文格式：钱娟娟,王克芬,刘胜利,等.产碱性纤维素酶菌株WS-135的筛选及酶学性质研究[J].食品与发酵科技,2020,56(5):60-63,71.