付阳洋¹，杨 敏^1*，汤陆扬¹，杨培昕¹，彭黔荣²，谢有超³

（1.贵州大学药学院，贵州贵阳 550025；

2.贵州中烟工业有限责任公司技术中心，贵州贵阳 550009；

3.贵州大学化学与化工学院，贵州贵阳 550025）

摘要：以无籽刺梨果实为原料，采用热水浸提法提取多糖。在单因素试验的基础上通过响应面对提取工艺条件优化，并进一步研究了其抗氧化活性。结果表明，无籽刺梨多糖提取工艺参数以浸提温度80 ℃、料液比30∶1 mL/g、浸提时间3 h为宜，实际多糖提取率8.37%。无籽刺梨多糖具有较好的抗氧化活性和一定的还原能力，对DPPH·和·OH清除的半抑制质量浓度（IC₅₀）分别为1.02 mg/mL和0.5 mg/mL。

关键词：无籽刺梨；多糖；热水浸提法；响应面；抗氧化活性

中图分类号：TQ281   文献标识码：A   文章编号：1674-506X（2021）02-0066-0007

Optimization of Extraction Process by Response Surface Methodology and Antioxidant Activity of Rose sterilis S.D.Shi Polysaccharides

FU Yang-yang¹, YANG Min^1*, TANG Lu-yang¹, YANG Pei-xin¹, PENG Qian-rong², XIE You-chao³

（1.School of Pharmacy, Guizhou University, Guiyang Guizhou 550025, China;

2.Technical Center of China Tobacco Guizhou Industry Co., Ltd., Guiyang Guizhou 550009, China;

3.School of Chemistry and Chemical Engineering, Guizhou University, Guiyang Guizhou 550025, China）

Abstract：Using Rose sterilis S.D.Shi fruit as raw material, polysaccharides are extracted by hot water extraction. On the basis of single factor experiments, the extraction process conditions are optimized through response faces. And further studied its antioxidant activity. The results showed that the optimum extraction process parameters of Rose sterilis S.D.Shi polysaccharides were extraction temperature of 80 ℃, material-to-liquid ratio of 30∶1 mL/g, and extraction time of 3 h. The actual extraction rate of polysaccharides was 8.37%. Rose sterilis S.D.Shi Pear polysaccharide has good antioxidant activity and certain reducing ability, and the semi-inhibitory mass concentration (IC50) for DPPPH · and · OH removal are 1.02 mg/mL and 0.5 mg/mL, respectively.

Keywords：Rose sterilis S.D.Shi; polysaccharide; hot water extraction method; response surface; antioxidant activity

doi：10.3969/j.issn.1674-506X.2021.02-009

收稿日期：2020-07-23

基金项目：国家自然科学基金项目（21562014）；贵州中烟科技项目（黔烟工技［2015］8号）

作者简介：付阳洋（1996-），男，硕士研究生。研究方向：功能保健制剂的研究。

*通信作者

引文格式：付阳洋,杨敏,汤陆扬,等.响应面优化无籽刺梨多糖提取工艺及抗氧化活性研究[J].食品与发酵科技,2021,57(2):66-72.