李丽鑫1，肖智超2，赵云龙1，王 蕊1，马文思1，王文浩3，代佳和2*，刘发山4*
（1.昆明市食品药品检验所，昆明 650000；
2.云南农业大学食品科学技术学院，昆明 650201；
3.红塔烟草（集团）有限责任公司，玉溪 653100；
4.弥渡县职业技术教育中心，大理 671000）
摘要：建立同时测定肉及肉制品中9种水溶性人工合成着色剂含量的固相萃取-高效液相色谱法。方法采用氨水-乙腈-水溶液（1∶70∶29，v/v/v）提取，固相萃取小柱富集净化，多波长检测分析。肉及肉制品中9种水溶性人工合成着色剂在0.1～10 μg/mL浓度内线性关系良好，R2＞0.999 9，检出限为0.02～0.20 mg/kg，回收率为75.0%～103.3%，相对标准偏差（RSD）为0.10%～1.73%。研究结果表明，本方法前处理操作简单，各种色素分离度和回收率高，检测结果准确，且实验重现性好。
关键词：固相萃取；高效液相色谱法；肉及肉制品；人工合成着色剂
中图分类号：TS207.3 文献标识码：A 文章编号：1674-506X（2024）03-0112-0005
Determination of 9 Synthetic Colorants in Meat and Meat Products by Solid Phase Extraction-High Performance Liquid Chromatography
LI Lixin1, XIAO Zhichao2, ZHAO Yunlong1, WANG Rui1, MA Wensi1, WANG Wenhao3, DAI Jiahe2*, LIU Fashan4*
（1.Kunming Institute for Food and Drug Control, Kunming 650000, China;
2.College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
3.Hongta Tobacco Group Co., Ltd., Yuxi 653100, China;
4.Midu County Vocational and Technical Education Center, Dali 671000, China）
Abstract：A solid phase extraction-high performance liquid chromatography method was established for the simultaneous determination of nine water- oluble synthetic colorants in meat and meat products. The method used extraction with ammonian-acetonitrile-aqueous solution (1∶70∶29, v/v/v), solid phase extraction column for enrichment and cleanup, and multi-wavelength detection to analyze. In meat and meat products, nine water-soluble synthetic colorants had good linear relationships at the concentrations of 0.1～10 μg/mL, the correlation coefficient R2＞0.999 9, the limits of detection were 0.02～0.20 mg/kg, the recoveries were 75.0%～103.3%, and the relative standard deviations (RSD) were 0.10%～1.73%. The results showed that the method was simple in pre-treatment, high in the separation and recovery of various pigments, accurate in detection results, and good in experimental reproducibility.
Keywords：solid phase extraction; high performance liquid chromatography; meat and meat products; synthetic colorant
doi：10.3969/j.issn.1674-506X.2024.03-018
收稿日期：2023-03-16
基金项目：2020年度云南省博士后科研基金资助项目
作者简介：李丽鑫（1987-），女，本科，工程师。研究方向：食品质量与安全。
*通信作者：代佳和（1992-），女，博士 ，讲师。研究方向：食品资源与营养工程；刘发山（1985-），男，硕士，工程师。研究方向：食品质量与安全。
引用格式：李丽鑫,肖智超,赵云龙,等.固相萃取-高效液相色谱法测定肉及肉制品中9种人工合成着色剂的研究[J].食品与发酵科技,2024,60(3):112-116.
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