房夕杰,李天罡,王松江,郭传庄,王建彬*,李小双,王 硕,宋 佳,王长海,连 战
(东晓生物科技股份有限公司,潍坊 262218)
摘要:该文对经大肠杆菌发酵得到L-缬氨酸发酵液进行初步膜过滤,后使用活性炭进行脱色,以脱色率和L-缬氨酸保留率评价脱色效果。考察了4种不同型号的活性炭对L-缬氨酸的脱色效果,实验表明,选择元力HPC-07活性炭为L-缬氨酸脱色活性炭,脱色条件为活性炭用量2%、脱色温度65 ℃、发酵液pH 5.5、脱色时间30min。发酵液颜色由棕黄色变为澄清无色,且L-缬氨酸保留率高于98.0%,上述研究为企业生产高质量L-缬氨酸提供数据参考。
关键词:L-缬氨酸;脱色工艺;活性炭
中图分类号:TS202.3 文献标识码:A 文章编号:1674-506X(2024)04-0020-0005
Study on Optimization of Activated Carbon Decolorization Process of L-valine Fermentation Broth
FANG Xijie,LI Tiangang,WANG Songjiang,GUO Chuanzhuang,WANG Jianbin*,LI Xiaoshuang,WANG Shuo,SONG Jia,WANG Changhai,LIAN Zhan
(Dongxiao Biotechnology Co.,Ltd.,Weifang 262218,China)
Abstract:In this paper,the L-valine fermentation broth obtained by Escherichia coli fermentation was preliminarily filtered by membrane filtration, followed by decolorization using activated carbon, and the decolorization effect was evaluated by decolorization rate and L-valine retention rate. The decolorization effect of four different types of activated carbon on L- valine was investigated, and the experiment determined that the Yuanli HPC- 07 activated carbon was selected as the decolorizing activated carbon, and the decolorization conditions were activated carbon dosage of 2%,decolorization temperature 65 ℃,fermentation broth pH 5.5,and decolorization time of 30 min. The color of the fermentation liquid was changed from brownish-yellow to a clarified colorless,and the retaining rate of L- valine was higher than 98.0% . The above research provides data reference for enterprises to produce high-quality L-valine.
Keywords:L-valine;decolorization process;active carbon
doi:10.3969/j.issn.1674-506X.2024.04-004
收稿日期:2023-09-28
基金项目:国家重点研发计划资助(2023YFD1300700)
作者简介:房夕杰(1993-),男,硕士,工程师。研究方向:提纯工艺研究。
*通信简介:王建彬(1987-),男,硕士,高级工程师。研究方向:微生物发酵控制及研发。
引用格式:房夕杰,李天罡,王松江,等.L-缬氨酸发酵液活性炭脱色工艺优化研究[J].食品与发酵科技,2024,60(4):20-24.
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