吕志飞¹，张志伟²，李天罡¹，王松江¹，郭传庄¹，隋松森¹，连 战¹，孙瑞成¹，董红梅¹，王建彬^1，2*

（1.东晓生物科技股份有限公司，潍坊262218；2.东晓生物工程（山东）有限公司，济南250300）

摘要：全细胞体系制备D-阿洛酮糖因成本低、工艺简单受到人们的关注，研究全细胞转化D-果糖为D-阿洛酮糖的较适条件对促进D-阿洛酮糖的产业化具有重要意义。该文探究了全细胞酶的储藏稳定性并对全细胞制备D-阿洛酮糖的条件（培养时间、细胞浓度、转化初始pH、转化温度、金属离子）进行了优化与验证。结果表明：全细胞酶具有较强的储藏稳定性，40℃放置48h催化效果未明显降低；在500g/L D-果糖底物浓度下，适用于生产的全细胞转化较适条件为细胞浓度10 g/L、转化温度70 ℃、转化初始pH为自然pH、转化率为31.55%，较优化前转化率提高约3%，转化周期缩短至4h，生产工序简化、生产效率提高、生产成本降低。该研究旨在为D-阿洛酮糖工业化生产提供实验基础，满足大规模生产的需求。

关键词：D-阿洛酮糖；全细胞；条件优化；转化率；工业化

中图分类号：TS245.9 文献标识码：A 文章编号：1674-506X（2024）05-0023-0007

Optimization of Conditions for Whole Cell Transformation to Produce D-allulose

LV Zhifei¹，ZHANG Zhiwei²，LI Tiangang¹，WANG Songjiang¹，GUO Chuanzhuang¹，SUI Songsen¹，

LIAN Zhan¹，SUN Ruicheng¹，DONG Hongmei¹，WANG Jianbin^1，2*

（1.Dongxiao Biotechnology Co.，Ltd.，Weifang 262218，China；2.Dongxiao Bioengineering（Shandong） Co.，Ltd.，Jinan 250300，China）

Abstract：The production of D-allulose in a whole cell system has attracted attention due to its low cost and simple process. Studying the suitable conditions for the conversion of D-fructose into D-allulose in whole cells is of great significance in promoting the industrialization of D-allulose. This article explored the storage stability of whole cell enzymes as well as optimized and validated the conditions for the production of D-allulose from whole cells （including culture time， cell concentration， initial conversion pH， conversion temperature， and metal ions） . The results showed that the whole cell enzyme had relatively strong storage stability， and the catalytic effect did not significantly decrease when placed at 40℃ for 48h；At a concentration of 500g/L D-fructose substrate，the suitable conditions for whole cell transformation in production were cell concentration of 10 g/L， conversion temperature of 70 ℃，initial conversion pH equaling to natural pH，and conversion rate up to 31.55%，which was about 3% higher than before optimization. The conversion cycle was shortened to 4 h，and the production process was simplified，production efficiency was improved，and production costs were reduced. The research aims to provide an experimental basis for the industrial production of D-allulose and meet the needs of large-scale production.

Keywords：D-allulose；whole cell；condition optimization；conversion rate；industrialization

doi：10.3969/j.issn.1674-506X.2024.05-004

收稿日期：2023-12-22

基金项目：山东省重点研发计划（重大科技创新工程）（2020CXGC010603）

作者简介：吕志飞（1992-），女，硕士，工程师。研究方向：微生物发酵控制及研发。

*通信作者：王建彬（1987-），男，硕士，高级工程师。研究方向：微生物发酵控制及研发。

引用格式：吕志飞，张志伟，李天罡，等.全细胞转化生产D-阿洛酮糖条件优化［J］.食品与发酵科技，2024，60（5）：23-29.