刘依欣^1，2，廖郑悦¹，李耀光³，高廷会⁴，王松涛¹，黄张君¹，刘思静²，沈才洪^1*，国锦琳^2，4*

（1.泸州品创科技有限公司/国家固态酿造工程技术研究中心，泸州646000；2.成都中医药大学医学技术学院，成都610041；3.成都中医药大学临床医学院，成都610041；4.成都中医药大学药学院/现代中药产业学院，成都610041）

摘要：探究绿豆大曲的活性成分及其对急性醉酒小鼠乙醇代谢和肝脏的具体影响与机制。通过超高压液相色谱串联四级杆飞行时间质谱（UPLC-Q-TOF-MS）技术分析绿豆大曲中潜在活性成分；建立急性醉酒小鼠模型，采用行为学实验、酶联免疫吸附实验（Enzyme linked immunosorbent assay, ELISA）、实时荧光定量聚合酶链反应（Real-time quantitative polymerase chain reaction, RT-qPCR）、自动生化分析技术探究绿豆大曲及活性成分对小鼠乙醇代谢和肝脏的具体影响；采用网络药理学技术结合蛋白免疫印迹实验（Western blot, WB）进一步探究绿豆大曲影响急性酒精性肝病的可能机制。结果显示，绿豆大曲存在32种潜在活性成分，具有促进小鼠乙醇代谢和醒酒的作用潜力，可减轻小鼠饮酒带来的肝脏负担，例如氧化应激、血脂紊乱、转氨酶升高及炎症因子分泌增多，这可能与其中活性成分协同抑制PI3K/AKT/MAPK信号通路相关。

关键词：绿豆大曲；急性醉酒；乙醇代谢；液相色谱-质谱；网络药理学

中图分类号：TS262.91 文献标识码：A 文章编号：1674-506X（2025）01-0001-0015

The Study on the Effect and Mechanism of Lvdou Daqu Active Components on Ethanol Metabolism and Liver Injury in Acute Intoxicated Mice

LIU Yixin^1，2，LIAO Zhengyue¹，LI Yaoguang³，GAO Tinghui⁴，WANG Songtao¹，HUANG Zhangjun¹， LIU Sijing²，SHEN Caihong^1*，GUO Jinlin^2，4*

（1.Luzhou Pinchuang Technology Co.，Ltd./National Engineering Research Center of Solid-state Brewing，Luzhou 646000，China；2.School of Medical Technology，Chengdu University of Traditional Chinese Medicine，Chengdu 610041，China；3.Schoole of Clinical Medicine，Chengdu University of Traditional Chinese Medicine，Chengdu 610041，China；4.School of Pharmacy/School of Modern Chinese Medicine Industry，Chengdu University of Traditional Chinese Medicine，Chengdu 610041，China）

Abstract: To investigate the active components of Lvdou Daqu and their specific effects and mechanisms on ethanol metabolism and liver in acute intoxicated mice. The potential active components in Lvdou Daqu were analyzed by Ultra-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry （UPLC-QTOF-MS）; a mouse model of acute intoxication was established, and behavioral experiments, enzyme linked immunosorbent assay （ELISA）, real-time quantitative polymerase chain reaction （RT-qPCR）, and automated biochemical analysis techniques were used to study the specific effects of Lvdou Daqu on ethanol metabolism and the liver. The possible mechanisms of Lvdou Daqu affecting acute alcoholic liver disease were further investigated using network pharmacology technique combined with western blot assay （WB） . The results showed the presence of 32 potential active components in Lvdou Daqu, and they had the potential effect to promote mice’s metabolism of ethanol and sobriety and could reduce the burden of the liver caused by alcohol consumption, such as oxidative stress, dyslipidemia, elevated aminotransferases, and increased secretion of inflammatory factors, that may be related to the synergistic inhibition of PI3K/AKT/MAPK signaling pathway from active components.

Keywords：Lvdou Daqu；acute intoxication；ethanol metabolism；LC-MS；network pharmacology

doi：10.3969/j.issn.1674-506X.2025.01-001