张黎梅1，汪云飞2，陈 艳1，董天秋1，马文思1，陈娴1*
（1.昆明市食品药品检验所（昆明市食品药品检验研究院），昆明650032；2.昆明市五华区疾病预防控制中心，昆明650000）
摘要：建立一种高效液相色谱法测定养生茶中双氯芬酸钠的方法。以80%甲醇为溶剂，超声（200W）提取20min，0.02mol/L乙酸铵＋0.05%甲酸溶液∶乙腈＝40∶60（v/v）为流动相，检测波长230nm，流速1.0mL/min，柱温35℃，进样量10 μL，外标法定量。检出限5.350 mg/kg，线性范围1.02～204 μg/mL，相关系数（R2）为0.999 9，加标回收率在77.0%～106.7%之间，相对标准偏差（RSD）在2%以内。该方法简单、准确、快速，与液质联用仪相比，该法成本低，普及率高，适用于养生茶中双氯芬酸钠的测定。
关键词：双氯芬酸钠；高效液相色谱法；养生茶
中图分类号：TS272 文献标识码：A 文章编号：1674-506X（2025）01-0169-0004
Determination of Diclofenac Sodium in Health Tea by HPLC-DAD Method
ZHANG Limei1，WANG Yunfei2，CHEN Yan1，DONG Tianqiu1，MA Wensi1，CHEN Xian1*
（1.Kunming Food and Drug Inspection Institute（Kunming Institute of Food and Drug Inspection and Research），Kunming 650032，China；2.Disease Prevention and Control Center of Wuhua District，Kunming，Kunming 650000，China）
Abstract：A high-performance liquid chromatography method was established for the determination of diclofenac sodium in health tea. Using 80% methanol as solvent, 200 W ultrasonic extraction for 20 min, 0.02 mol/L ammonium acetate+0.05% formic acid solution∶acetonitrile=40:60 (v/v) as mobile phase, detection wavelength of 230 nm, flow rate of 1.0 mL/min, column temperature of 35℃, injection volume of 10 μL, external standard method was used for quantitative analysis. Detection limit was 5.350 mg/kg, linear range was 1.02~204 μg/mL, correlation coefficient (R2) was 0.999 9, spiked recovery rate was between 77.0%~106.7%, relative standard deviation (RSD) was within 2%. This method is simple, accurate, and fast. Compared with liquid chromatography-mass spectrometry, this method has lower cost and higher popularity rate, suitable for the determination of diclofenac sodium in health tea.
Keywords：diclofenac sodium；high performance liquid chromatography；health tea
doi：10.3969/j.issn.1674-506X.2025.01-023
