李建湘，丁红梅*，姜敬维，朱洪日*，韩 辉

（陆军勤务学院，重庆401331）

摘要：该文以枸杞提取物、壳寡糖、沙棘提取物、银杏提取物为研究对象，利用真空机和低压氧舱建立小鼠缺氧疲劳模型，通过单因素实验和正交实验，得到最佳组方为枸杞提取物添加量15g、壳寡糖添加量17.5g、沙棘提取物添加量15g和银杏提取物添加量10g。通过喂食最佳组方，发现与对照组相比，游泳时间延长了1.25倍；进行缺氧疲劳实验后发现，乳酸脱氢酶（LDH）、超氧化物歧化酶（SOD）的含量分别是对照组的1.79倍、1.49倍，肝糖原含量是对照组的1.40倍，血乳酸（BLA）和血尿素氮（BUN）的含量分别是对照组的0.59倍和0.84倍，丙二醛（MDA）水平是对照组的0.71倍，进行低压氧舱跑台实验后，发现乳酸脱氢酶（LDH）含量是对照组的1.75倍，血氧饱和度（SaO2）、血乳酸（BLA）分别是对照组的1.12倍、0.54倍，实验结果表明该组方可延长小鼠在缺氧疲劳环境下的运动时长。

关键词：抗疲劳；缺氧；药食同源

中图分类号：R285 文献标识码：A 文章编号：1674-506X（2025）01-0108-0006

Fatigue Resist Formula Improvement and Its Effect on Mice

LI Jianxiang，DING Hongmei*，JIANG Jingwei，ZHU Hongri*，HAN Hui

（Army Logistics Academy，Chongqing 401331，China）

Abstract： In this article, with Lycium barbarum extract, chitosan oligosaccharide, sea buckthorn extract and ginkgo extract as the research objects, a vacuum machine and a hypobaric oxygen chamber were used to establish a mouse hypoxic fatigue model. Through single factor and orthogonal experiments, the optimal formula addition obtained was Lycium barbarum extract 15 g, chitosan oligosaccharide 17.5 g, sea buckthorn extract 15 g and ginkgo extract 10 g. By feeding with the optimal formula, it was found that compared with the control group the swimming time increased by 1.25 times; through hypoxia and fatigue experiments, lactate dehydrogenase (LDH)and superoxide dismutase (SOD) were respectively 1.79 and 1.49 times that of the control group, and liver glycogen content was 1.40 times that of the control group. Blood lactate (BLA) and blood urea nitrogen (BUN) were 0.59 times and 0.84 times that of the control group, respectively, while level of malondialdehyde (MDA) was 0.71 times that of the control group. After conducting a low-pressure oxygen chamber treadmill experiment, it was found that compared to the control group, LDH content increased by 1.75 times, blood oxygen saturation (SaO2) increased by 1.12 times, and BLA was 0.54 times that of the control group. The experiment results indicated that this treatment can prolong the exercise duration of mice in a hypoxic fatigue environment.

Keywords：fatigue resist；hypoxia；function as both medicine and food

doi：10.3969/j.issn.1674-506X.2024-002