李艳军，赖理智，李 荔*

（佛山市海天（高明）调味食品有限公司，佛山528500）

摘要：该文以米曲霉为研究对象，通过单因素试验和正交试验，确定了米曲霉固体培养的最佳条件。试验结果表明，最佳组合为玉米粉添加量为20%、大豆蛋白添加量为10%、农作物秸秆＋麸皮添加量为65%、培养温度为34～36 ℃、培养时间为72 h、培养基起始含水量控制在55%，在此条件下米曲霉的蛋白酶酶活力达到13 400 U/g、孢子数为130.5亿个/g，发芽率为92%。采用优化后的种曲进行圆盘制曲培养，优化组的蛋白酶酶活力平均值为3 376U/g、孢子数平均值为2.6亿个/g，较对照组分别提升了18.2%和30%。

关键词：米曲霉；碳源；氮源；正交试验

中图分类号：TS201.3；TS264.21 文献标识码：A 文章编号：1674-506X（2025）06-0047-0009

Study on the Components and Cultivation Conditions of Solid-state Medium for Aspergillus oryzae Used in Soy Sauce Production

LI Yanjun，LAI Lizhi，LI Li*

（Foshan Haitian （Gaoming） Flavoring & Food Co.，Ltd.，Foshan 528500，China）

Abstract: This paper takes Aspergillus oryzae as the research object，and determines the optimal conditions for solid-state cultivation of Aspergillus oryzae through single factor experiment and orthogonal experiment. The results showed that the optimal combination was 20% corn flour adding amount， 10% soybean protein adding amount， 65% crop straw＋ bran adding amount， 34～36 ℃ culture temperature， 72 h culture time， and 55% initial water content of culture medium. Under these conditions， the protease activity of Aspergillus oryzae reached 13 400 U/g，the number of spores was 13.05 billion/g，and the germination rate was 92%. The optimized seed koji was used for disc culture，and the average protease activity and spore number of the optimized group were 3 376 U/g and 260 million/g，which increased by 18.2% and 30%，respectively，compared to the control group.

Keywords: Aspergillus oryzae；carbon sources；nitrogen sources；orthogonal experiment

doi: 10.3969/j.issn.1674-506X.2025.06-007