李嘉俊^1，2，黄燕秋^1，2，苏汝彬^1，2，赵 斌^1，2*，彭荣珍^1，2，蔡佳梓^1，2，吴嘉燕^1，2，黄方喆¹，文艺璇¹

（1.广东江门中医药职业学院/国家中药现代化工程技术研究中心江门健康产品分中心，江门529000；

2.江门市健康产品工程技术研究中心，江门529000）

摘要：通过以纤维素酶对簕菜进行处理，并采用大孔吸附树脂加以纯化，制备出一种簕菜提取物。为了研究簕菜提取物中的活性成分含量，以及簕菜提取物的体外抗氧化活性，该研究采用高效液相色谱法对簕菜提取物中的绿原酸、隐绿原酸、芦丁、异绿原酸A、异绿原酸B、异绿原酸C等多酚类物质进行测定，并评价了簕菜提取物对DPPH自由基和ABTS＋自由基的清除能力。结果表明，簕菜提取物中6种活性成分的总含量为31.76%，其中绿原酸的含量最高；簕菜提取物对DPPH自由基和ABTS＋自由基清除能力的IC50值分别为13.91μg/mL、26.22μg/mL，具有良好的抗氧化活性。该研究为簕菜的开发利用提供了理论依据。

关键词：簕菜；提取物；含量；体外抗氧化活性

中图分类号：TS255.1 文献标识码：A 文章编号：1674-506X（2025）06-0119-0006

Study on the Content of Active Components in the Extract of Acanthopanax trifoliatus and Its in Vitro Antioxidant Activity

LI Jiajun^1，2，HUANG Yanqiu^1，2，SU Rubin^1，2，ZHAO Bin^1，2*，PENG Rongzhen^1，2，CAI Jiazi^1，2，WU Jiayan^1，2，HUANG Fangzhe¹，WEN Yixuan¹

（1.Guangdong Jiangmen Chinese Medicine College/Jiangmen Health Products Branch Center，National Engineering Technology Research Center for Modernization of Traditional Chinese Medicine，Jiangmen 529000，China；

2.Jiangmen Engineering Technology Research Center of Health Products，Jiangmen 529000，China）

Abstract: By treating Acanthopanax trifoliatus with cellulase and purifying it using macroporous adsorption resin， an extract of Acanthopanax trifoliatus has been prepared. In order to study the content of active components in the extract of Acanthopanax trifoliatus and evaluate its in vitro antioxidant activity，in this study，high performance liquid chromatography （HPLC） was used to determine the content of chlorogenic acid （CA），4-dicaffeoylquinic acid （4DA），rutin （Rut），isochlorogenic acid A （IAA），isochlorogenic acid B （IAB），isochlorogenic acid C （IAC） in the extract of Acanthopanax trifoliatus. The ability of the extract of Acanthopanax trifoliatus to scavenge DPPH and ABTS＋ free radicals was studied. The results showed that the total content of 6 active components in the extract of Acanthopanax trifoliatus was 31.76%， of which chlorogenic acid had the highest content； The IC50 values of the DPPH free radical and ABTS＋ free radical scavenging abilities of the extract from Acanthopanax trifoliatus were 13.91 μg/mL and 26.22 μg/mL，respectively，exhibiting good antioxidant activity. This study provides theoretical basis for the development and utilization of the extract of Acanthopanax trifoliatus.

Keywords: Acanthopanax trifoliatus；extract；content；in vitro antioxidant activity

doi: 10.3969/j.issn.1674-506X.2025.06-017