谭芸芸，谭书明^*，俞露，任廷远，谢国芳，陈超，徐青，袁梦，陈珍

（贵州大学酿酒与食品工程学院，贵州贵阳 550025）

摘要：为了探究刺梨鞣花单宁对db/db小鼠肝脏代谢紊乱的影响。将C57/BKS-db/m小鼠设为空白组，18只C57/BKS-db/db小鼠按体重随机分为模型组，阳性对照组（盐酸二甲双胍灌胃剂量50 mg/kg），鞣花单宁组（刺梨鞣花单宁灌胃剂量50 mg/kg），每组6只，实验时间8周；实验结束后检测各组实验小鼠肝脏的丙酮酸激酶（PK）、己糖激酶（HK）和葡萄糖-6-磷酸脱氢酶（G6PD）水平；采用全局精准非靶向代谢技术分析实验小鼠肝脏差异代谢物及信号通路。结果表明，与模型组相比，刺梨鞣花单宁可以显著（P＜0.05）增加HK、PK水平，显著（P＜0.05）降低小鼠的G6PD水平；HE染色发现，刺梨鞣花单宁显著改善肝脏的病变程度。LC-MS检测进一步发现，刺梨鞣花单宁干预后，实验小鼠肝脏中筛选出154个差异代谢物，其中106个差异代谢物上调，48个差异代谢物下调。KEGG富集分析表明，在鞣花单宁干预下，大量差异代谢物显著（P＜0.05）富集在mTOR信号通路、蛋白质消化吸收、赖氨酸降解、亚油酸代谢、矿物质吸收、5-羟色胺能突触、泛酸和辅酶A生物合成等通路上。刺梨鞣花单宁可能在焦谷氨酸、色氨酸和犬尿氨酸的联合作用下改善了db/db小鼠的代谢紊乱，并通过精氨酸抑制mTOR下游通路，从而促进自噬，达到改善胰岛素抵抗。刺梨鞣花单宁对维持2型糖尿病葡萄糖脂稳态上具有潜在价值，为进一步开发功能性产品提供理论支撑。

关键词：液相色谱-质谱法；刺梨鞣花单宁；糖尿病

中图分类号：TS255.1      文献标识码：A       文章编号：1674-506X（2023）01-0022-0009

Effect of Ellagitannin of Rosa roxburghii Tratt on db/db Mice was Analyzed Based on Metabolomics

TAN Yunyun, TAN Shuming^*, YU Lu, REN Tingyuan, XIE Guofang, CHEN Chao, XU Qing，

YUAN Meng, CHEN Zhen

（School of Liquor and Food Engineering, Guizhou University, Guiyang Guizhou 550025, China）

Abstract: To investigate the effect of ellagitannin from Rosa roxburghii Tratt on hepatic metabolism in db/db mice. C57/BKS-db/m mice were set as a blank group, and 18 C57/BKS-db/db mice were divided randomly into the model group, positive control group (guanidine dicarboxylate hydrochloride gavage dose 50 mg/kg), ellagitannin group (ellagitannin from Rosa roxburghii Tratt gavage dose 50 mg/kg) according to body weight, 6 mice in each group for 8 weeks. The levels of pyruvate kinase (PK), hexokinase(HK), and glucose-6-phosphate dehydrogenase (G6PD) were measured in the liver of each group of experimental mice at the end of the experiment. Differential metabolites and signaling pathways in mice livers were analyzed by global precision untargeted metabolism. The results showed that compared with the model group, ellagitannin significantly (P＜0.05) increased the levels of HK and PK,and significantly (P＜0.05) decreased the levels of G6PD in mice. HE staining showed that ellagitannin in Rosa roxburghii Tratt significantly improved the degree of liver lesion.

Keywords: liquid chromatography-mass spectrometry (LC-MS); ellagitannin from Rosa roxburghii Tratt; diabetes mellitus

doi: 10.3969/j.issn.1674-506X.2023.01-004

收稿日期：2022-09-16

基金项目：贵州省刺梨产业研究院平台建设（黔财农（2019）261号）

作者简介：谭芸芸（1997-），女，硕士研究生。研究方向：食品加工安全。

*通信作者：谭书明（1964-），男，教授。研究方向：食品科学。

引用格式:谭芸芸,谭书明,俞露,等.基于代谢组学分析刺梨鞣花单宁对db/db小鼠的影响[J].食品与发酵科技,2023,59(1):22-29,44.