李文孝^1,2，梁建强¹，贾俊超¹，赵文娟^1*，秦 涛²，冯璞阳¹

（1.陕西省生物农业研究所，陕西西安 710043；2.陕西省酶工程技术研究中心，陕西西安 710600）

摘要：探索极端东方化假单胞菌SWNY 3-6产嗜铁素的发酵条件，旨在为嗜铁素的深入应用研究及其在农业生物防治领域的推广应用提供数据支撑。本研究以嗜铁素相对含量为评价指标，在培养温度、发酵时间、pH、通气量单因素实验基础上，利用Box-Behnken中心组合原则，设计四因素三水平响应面实验，对其发酵条件进行优化。结果表明，优化后的发酵条件为培养温度35.5℃、发酵时间38.5 h、pH值7.2、通气量1.8 vvm，在此条件下嗜铁素相对含量达到了59.36%，接近模型预估值。通过响应面法优化极端东方化假单胞菌产嗜铁素的发酵条件，确定产嗜铁素的最优发酵条件，为后续生产及应用研究提供理论依据。

关键词：极端东方化假单胞菌SWNY 3-6；嗜铁素；发酵条件；响应面法

中图分类号：Q815    文献标识码：A      文章编号：1674-506X（2023）01-0108-0006

Optimization of the Fermentation Condition for Siderophores Production 

by Pseudomonas extremorientalis by Response Surface Methodology

LI Wenxiao^1,2, LIANG Jianqiang¹, JIA Junchao¹, ZHAO Wenjuan^1*, QIN Tao², FENG Puyang¹

(1.Bio-Agriculture Institute of Shaanxi, Xi’an Shaanxi 710043, China;

2.Enzyme Engineering Research Center of Shaanxi Province, Xi’an Shaanxi 710600, China)

Abstract: In this study, we explored the fermentation conditions of Pseudomonas extremorientalis SWNY3-6 for siderophores production, aiming to provide data support for the further application of siderophores and its popularization and application in the agricultural biological control field. Relative content of siderophores was applied as the evaluation index. Firstly, the separate experiments of culture temperature, fermentation time, pH and ventilation was conducted, respectively. Then the response surface methodology was designed with four factors and three levels to optimize the fermentation conditions, using the Box- Behnken central combination principle. The obtained optimal fermentation conditions were 38.5 h fermentation at 35.5 ℃, with a pH value of 7.2 and ventilation 1.8 vvm. Under these conditions, the relative content of siderophores reached 59.36%, close to the predicted value of the model. The optimal fermentation conditions for siderophores production by Pseudomonas extremorientalis were determined, which provided a theoretical basis for subsequent production and application research.

Keywords: Pseudomonas extremorientalis SWNY3- 6; siderophores; fermentation condition; response surface method

doi: 10.3969/j.issn.1674-506X.2023.01-015

收稿时间：2022-08-23

基金项目：陕西省重点产业创新链（2021ZDLNY05-01）；陕西省科学院重大科学研究专项（2018K-06）；陕西省重点研发计划（2019NY-191）

作者简介：李文孝（1977-），男，硕士，副研究员。研究方向：农业微生物菌剂、肥料开发与应用研究。

*通信作者：赵文娟（1972-），女，研究员。研究方向：农用微生物与有机肥综合研究。

引用格式:李文孝,梁建强,贾俊超,等.响应面法对极端东方化假单胞菌产嗜铁素发酵条件的优化[J].食品与发酵科技,2023,59(1):108-112,139.