李丽娜，李晶，李东红，余萍，矫艳平*，陈雪娇

（江西仁仁健康微生态科技有限公司，樟树  331200)

摘要：本文研究了由姜、红糖、大枣和低聚异麦芽糖为原料生产的老姜酵素对缓解由寒凝血瘀引起的不良反应的作用效果。通过建造寒凝血瘀大鼠模型，同步灌胃老姜酵素，分为低剂量组、中剂量组和高剂量组，观察大鼠的体重等基本特征、扭体反应，测定服用前后的子宫、卵巢和肾脏的脏器指数，血液流变学特性，分析其PGF2α和PGE2数值变化，Ca²⁺超载量和氧化应激反应。结果表明，与模型组相比，老姜酵素的低剂量组、中剂量组和高剂量组均改善大鼠的体重和基本指标，高剂量组的大鼠扭体反应次数明显下降，血液黏度降低，说明老姜酵素有助于缓解寒凝血瘀引起的不良反应且对健康没有影响。高剂量组大鼠的PGF2α、PGE2比值与模型组相比显著下降，改善Ca²⁺超载量，提升SOD酶活性。表明老姜酵素对寒凝血瘀症有一定的改善作用。

关键词：益生菌；老姜酵素；寒凝血瘀证

中图分类号：TQ925    文献标识码：A    文章编号：1674-506X（2024）02-0079-0007

Study on the Preparation of Old Ginger Enzyme by Probiotic Materia Medica Fermentation Technology and Its Effect on Cold Coagulation Blood Stasis Model in Rats

LI Lina, LI Jing, LI Donghong, YU Ping, JIAO Yanping*, CHEN Xuejiao

(Jiangxi Renren Health Micro Ecological Technology Co., Ltd., Zhangshu 331200, China)

Abstract: In this paper, the effect of old ginger enzyme produced from ginger, brown sugar, jujube and isomaltooligosaccharide on alleviating the adverse reactions caused by cold coagulation and blood stasis was studied. By establishing the rat model of cold coagulation and blood stasis, the rats were divided into three groups: low dose group, middle dose group and high dose group. Observe the basic characteristics of rats, such as body weight, writhing reaction, determine the organ index of uterus, ovary and kidney, and the hemorheological characteristics before and after taking it, and analyze the numerical changes of PGF2α and PGE2, Ca²⁺ overload and oxidative stress reaction. The results showed that compared with the model group, the low-dose group, middle-dose group and high-dose group improved the body weight and basic indexes of rats, and the writhing times and blood viscosity decreased significantly in the high-dose group, it shows that old ginger enzyme helps to alleviate the adverse reactions caused by cold coagulation and blood stasis and has no effect on health. The ratio of PGF2α to PGE2 in the high dose group was significantly lower than that in the model group, the overload of Ca²⁺ was improved and the activity of SOD enzyme was increased. It shows that old ginger enzyme can improve the syndrome of cold coagulation and blood stasis to some extent.

Keywords: probiotics; old ginger enzyme; cold coagulation blood stasis

doi: 10.3969/j.issn.1674-506X.2024.02-012

收稿日期：2023-05-22

作者简介：李丽娜（1990-），女，硕士，工程师。研究方向：生物发酵。

*通信作者：矫艳平（1989-），女，硕士，工程师。研究方向：益生菌制剂。

引用格式:李丽娜,李晶,李东红,等.益生菌老姜酵素改善大鼠寒凝血瘀的作用研究[J].食品与发酵科技,2024,60(2):79-84,100.