张朝志1,邓 巍1,杨晓燕1,2,3*,肖 文1,2,3
(1.大理大学东喜玛拉雅研究院,云南大理 671003;
2.中国三江并流区域生物多样性协同创新中心,云南大理 671003;
3.大理大学三江并流区域生物多样性保护与利用云南省创新团队,云南大理 671003)
摘要:本研究运用高通量测序技术对泡菜母水发酵60 d内的耐药基因进行监测,发现其中含有6种耐药基因,分别是β-内酰胺类、抗叶酸剂、万古霉素、阳离子抗菌肽、EGFR酪氨酸激酶抑制剂和铂类。通过分析不同发酵阶段泡菜母水中耐药基因的动态变化,结果发现:随着发酵天数的增加,泡菜母水中耐药基因的相对丰度有不同程度的增减;6种耐药基因与20个优势属之间具有一定的相关关系,其中与微小杆菌属(Exiguobacterium)和拉恩氏菌属(Rahnella)相关性较强。
关键词:食品安全;发酵;高通量测序;细菌耐药;抗生素
中图分类号:TS255.54 文献标识码:A 文章编号:1674-506X(2023)04-0058-0006
Bacteria-associated Drug Resistance Gene Types and Their Dynamic Changes in Paocai Brine
ZHANG Chaozhi1,DENG Wei1,YANG Xiaoyan1,2,3*,XIAO Wen1,2,3
(1.Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali Yunnan 671003, China;
2.Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China, Dali Yunnan 671003, China;
3.The Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region from Dali University, Dali Yunnan 671003, China)
Abstract:In this work, drug resistance genes in Paocai brine were tracked throughout the course of 60 days of fermentation using high throughput sequencing technologies. Beta-lactams, anti-folate medications, vancomycin, cationic antimicrobial peptides, EGFR tyrosine kinase inhibitors, and platinum were the six resistance genes discovered in total. The results demonstrated that as the number of fermentation days rose, the relative abundance of drug resistance genes fluctuated. Twenty dominant genus showed correlation with drug resistance genes, with Exiguobacterium and Rahnella showing the strongest correlation.
Keywords:food safety; fermentation; high-throughput sequencing; bacterial drug resistance; antibiotics
doi:10.3969/j.issn.1674-506X.2023.04-009
收稿日期:2022-11-09
基金项目:云南省科技厅基础研究地方高效联合基金面上项目(202101BA070001-115);云南省汪景琇院士工作站(202005AF150025)
作者简介:张朝志(2001-),女,本科生。研究方向:微生物学。
*通信作者:杨晓燕(1977-),博士,教授。研究方向:微生物学。
引用格式:张朝志,邓巍,杨晓燕,等.泡菜母水中的细菌相关耐药基因种类及其动态变化[J].食品与发酵科技,2023,59(4):58-63.
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