赵 丽²，徐晓琴¹，张 娟¹，卿德刚¹，刘天志³，孙 宇^1*

（1.新疆维吾尔自治区中药民族药研究所，新疆乌鲁木齐 830002；

2.新疆鸿昆生物科技有限公司，新疆昌吉 831100；

3.新疆和田阳光沙漠玫瑰有限公司，新疆和田 848000）

摘要：考察肉苁蓉玫瑰花口服液对小鼠免疫力的综合影响，评价该产品的增强免疫力效果，为其产品开发提供实验数据和理论基础。实验选用SPF级ICR种雌性小鼠，并按照实验方案划分为各个大组，每个大组依次设立对照组、肉苁蓉玫瑰花口服液低（3.33 mL/kg·bw）、中（6.67 mL/kg·bw）、高（20.00 mL/kg·bw）剂量组，连续灌胃给药30 d。各大组依次进行碳廓清实验；ConA诱发小鼠淋巴细胞转化实验（MTT法）；NK细胞的活力测定；脏体比值测定、半数溶血值（HC50）的检测和抗体产生细胞数量的检测；迟发型变态反应实验等实验。结果表明，与对照组相比，中、高剂量组可显著提高小鼠NK细胞活性、迟发型变态反应能力（P＜0.05）；高剂量能显著提高小鼠单核-巨噬细胞碳廓清能力、抗体生成细胞数、半数溶血值（P＜0.05）；各剂量对小鼠重量增长系数、脾脏/体重比值、胸腺/体重比值及淋巴细胞转化能力水平均无显著影响（P＞0.05）。按照保健食品检验与评价技术规范中判断标准，可以判定肉苁蓉玫瑰花口服液具有明显的增强免疫力的功效。

关键词：肉苁蓉玫瑰花口服液；增强免疫力；保健食品

中图分类号：TS218    文献标识码：A    文章编号：1674-506X（2023）05-0058-0006

Study on the Immune Enhancement Effect of Cistanche deserticola Rose Oral Liquid

ZHAO Li², XU Xiaoqing¹, ZHANG Juan¹, QING Degang¹, LIU Tianzhi³, SUN Yu^1*

（1.Xinjiang Institute of Chinese Materia Medica and Ethical Materia Medica, Urumqi Xinjiang 830002, China;

2.Xinjiang Hongkun Biotechnology Co., Ltd., Changji Xinjiang 831100, China;

3.Xinjiang Hetian Sunshine Desert Rose Co., Ltd., Hetian Xinjiang 848000, China）

Abstract：To investigate the comprehensive effect of Cistanche deserticola rose oral liquid on the immunity of mice, and to evaluate the effect of enhancing immunity of the product, so as to provide some experiment data and theory basis for its product development. SPF class ICR female mice were selected and divided into large groups according to experimental design, control group, Cistanche rose oral liquid low (3.33 mL/kg·bw), medium (6.67 mL/kg · bw) and high (20.00 mL/kg · bw) dose groups were set up in each group, and the administration was continued for 30 days. Carbon Clearance Test, ConA-induced lymphocyte transformation test (MTT method), activity of NK cells, ratio of viscera to body, half hemolysis value (HC50) and number of antibody-producing cells were measured. Delayed hypersensitivity test and other tests. Compared with the control group, the middle and high dose groups could significantly improve the ability of NK cell activity and delayed hypersensitivity (P＜0.05). High dose could significantly increase the carbon clearance capacity of mononuclear macrophages, the number of antibody producing cells and the half hemolysis value (P＜0.05). There were no significant effects on body weight gain, spleen-to-body weight ratio, thymus-to-body weight ratio and lymphocyte transformation ability at different doses (P＞0.05). According to the standard of health food test and evaluation, it was concluded that Cistanche deserticola rose oral liquid could enhance the immune function.

Keywords：Cistanche deserticola rose oral liquid; enhance immunity; health food

doi：10.3969/j.issn.1674-506X.2023.05-010

收稿日期：2023-01-10

基金项目：新疆维吾尔自治区二〇二一年（农业领域）重点研发项目“玫瑰功能性产品创制与应用”（2021B02005-4）

作者简介：赵丽（1990-），女，硕士，助理研究员。研究方向：食药产品开发。

*通信作者：孙宇（1985-），男，硕士，副研究员。研究方向：新疆特色资源基础研究及产品开发。

引用格式：赵丽,徐晓琴,张娟,等.肉苁蓉玫瑰花口服液增强免疫力作用的研究[J].食品与发酵科技,2023,59(5):58-63.