缪文玉¹，杨金梅²，宋露露²，周茹茹²，王辉敏²，秦 楠^2*

（1.太原城市职业技术学院管理系，山西太原 030027；

2.山西中医药大学中药与食品工程学院，山西晋中 030619）

摘要：初步探究火麻油对幽门螺杆菌（Helicobacter pylori，Hp）的抑菌活性及作用。通过二倍稀释法测定火麻油对Hp的抑菌圈直径、最小抑菌浓度（MIC）、最小杀菌浓度（MBC）、生长曲线及胞内核酸和蛋白质泄漏情况。结果表明，火麻油对Hp的抑菌圈直径为（16.51±0.08）mm，MIC为1/128 mL，MBC为1/32 mL。火麻油对Hp的生长具有明显的抑制作用，并使菌液中核酸和蛋白质含量均增加，且1 MIC的抑制作用明显优于1/2 MIC。研究结果表明火麻油对Hp具有抑制活性，初步推测其抑制作用可能是通过破坏细菌细胞壁而发挥的。

关键词：火麻油；幽门螺杆菌；抑菌活性；抑菌机理

中图分类号：R285   文献标识码：A   文章编号：1674-506X（2023）05-0023-0005

Study on Antibacterial Activity and Effect of Hemp Oil on Helicobacter pylori

MIAO Wenyu¹, YANG Jinmei², SONG Lulu², ZHOU Ruru², WANG Huimin², QIN Nan^2*

（1.Department of Management, Taiyuan City Polytechnic, Taiyuan Shanxi 030027, China;

2.College of Chinese Medicine and Food Engineering, Shanxi University of Traditional Chinese Medicine, Jinzhong Shanxi 030619, China）

Abstract：To explore the antibacterial activity and effect of hemp oil on Helicobacter pylori (Hp). The inhibition zone diameter, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), growth curve and intracellular nucleic acid and protein leakage of hemp oil against Helicobacter pylori were determined by double dilution method. The inhibition zone diameter of hemp oil against Hp was (16.51±0.08) mm, MIC was 1/128 mL, MBC was 1/32 mL. Hemp oil had a significant inhibitory effect on the growth of Hp, and increased the content of nucleic acid and protein in the bacterial solution, and the inhibitory effect of 1 MIC was significantly better than that of 1/2 MIC. Hemp oil has inhibitory activity against Hp, and it is preliminarily speculated that its inhibitory effect may be exerted by destroying bacterial cell wall.

Keywords：hemp oil; Helicobacter pylori; antibacterial activity; antibacterial mechanism

doi：10.3969/j.issn.1674-506X.2023.05-004

收稿时间：2022-12-03

基金项目：山西教育厅项目（2019L0729）

作者简介：缪文玉（1982-），女，硕士，讲师。研究方向：天然产物成分分析。

*通信作者：秦楠（1981-），男，博士，副教授。研究方向：食品微生物发酵及功能食品研究。

引用格式：缪文玉,杨金梅,宋露露,等.火麻油对幽门螺杆菌抑菌活性及作用研究[J].食品与发酵科技,2023,59(5):23-27.