QuEChERS-LC-MS/MS法快速检测鱼肉中54种兽药残留

1,吴彦蕾1,邓惠玲2,董宪兵1,陈 1,黄大亮1,周纯洁1*

1.重庆市食品药品检验检测研究院,国家市场监管重点实验室(调味品监管技术)

国家药品监督管理局麻醉精神药品质量监测重点实验室,重庆 401121

2.重庆市科学技术研究院,重庆401121

摘要:为改进和优化鱼肉中多兽残检测的QuEChERS样品前处理方法,考察了提取剂、吸附剂以及浓缩过程对54种兽药提取回收率的影响,建立了以乙腈饱和的正己烷为分散剂,0.5%甲酸乙腈为提取剂,C18粉为吸附剂并采用稀释法减少溶剂效应的QuEChERS样品前处理方法,结合液相色谱-串联质谱(LC-MS/MS)技术可同时测定鱼肉中54种兽药残留。方法简便、快速、准确、可靠,适用于鱼肉样品中多兽药残留的快速检测。

关键词:QuEChERS;兽药残留;液相色谱-串联质谱;鱼肉

中图分类号:TS207.3    文献标识码:A    文章编号:1674-506X202306-0135-0007


Rapid Determination of 54 Veterinary Drug Residues in Fish by QuEChERS-LC-MS/MS

SU Min1, WU Yanlei1, DENG Huiling2, DONG Xianbing1, CHEN Xin1, HUANG Daliang1, ZHOU Chunjie1*

1.Key Laboratory of Condiment Supervision Technology for State Market Regulation, NMPA Key Laboratory for Quality Monitoring of Narcotic Drugs and Psychotropic Substances, Chongqing Institute for Food and Drug Control, Chongqing 401121, China;

2.Chongqing Academy of Science and Technology, Chongqing 401121, China

AbstractIn order to optimize and improve QuEChERS method for sample pretreatment of determination of multiple veterinary drug residues in fish, the effects of extractant, adsorbent and concentration process on the extraction efficiencies of the 54 veterinary drugs were investigated. The high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection of the 54 veterinary drug residues was developed, using QuEChERS as the sample pretreatment method with the dispersant of acetonitrile saturated n-hexane, the extractant of 0.5% formic acid acetonitrile, the adsorbent of C18 powder, and the dilution operation to reduce solvent effects. This method is simple, rapid, accurate and reliable. It is suitable for the rapid detection of multiple veterinary drug residues in fish.

KeywordsQuEChERS; veterinary drug residues; high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS); fish

doi10.3969/j.issn.1674-506X.2023.06-023


收稿日期:2023-03-20

基金项目:重庆市市场监督管理局科研资助项目(CQSJKJ2020023);重庆市科研机构绩效激励引导专项(cstc2021jxjl00016

作者简介:敏(1992-),女,硕士,工程师。研究方向:食品安全与检测。

*通信作者:周纯洁(1985-),女,博士,高级工程师。研究方向:食品安全与检测。


引用格式:苏敏,吴彦蕾,邓惠玲,.QuEChERS-LC-MS/MS法快速检测鱼肉中54种兽药残留[J].食品与发酵科技,2023,59(6):135-141.


全文查看

公告通知

联系我们

  • 双月刊

  • 四川省经济和信息化厅

  • 四川省食品发酵工业研究设计院有限公司

  • 《食品与发酵科技》编辑部

  • 四川省成都市温江区杨柳东路中段98号

  • 611130

  • 028-82763572

  • ISSN1674-506X

  • CN51-1713/TS

  • 62-247