江 林^1，2，黄立森^1，2，付姗姗^1，2，王贵弘^1，2，庞海月^1，2*

（1.厦门医学院公共卫生与医学技术系，福建厦门 361023；

2.天然化妆品福建省高校工程研究中心，福建厦门 361023）

摘要：探究微生物发酵对铁皮石斛花功效活性的影响。分别将毕赤酵母、酿酒酵母、乳酸菌三株菌对铁皮石斛花进行纯种发酵，以80%乙醇为溶剂进行超声提取得到发酵铁皮石斛花乙醇提取物。测定其黄酮和总酚含量，通过DPPH、ABTS^＋自由基清除能力和总抗氧化能力测定，探究其发酵前后的抗氧化活性和抑制酪氨酸酶活性的美白功效。结果表明，经毕赤酵母发酵后的铁皮石斛花乙醇提取物中黄酮含量最高为13.17 mg/g，经酿酒酵母发酵铁皮石斛花乙醇提取物中总酚含量最高为1.04 mg/g。随着提取物浓度增大，提取物整体抗氧化能力逐渐增强，在1 mg/mL条件下，酿酒酵母发酵铁皮石斛花乙醇提取物的DPPH自由基清除率最高达81.5%，对ABTS＋自由基清除率最高为66.9%，总抗氧化能力最强为1.03 mmol/g，乳酸菌发酵铁皮石斛花乙醇提取物的酪氨酸酶抑制率最高为37.8%。

关键词：铁皮石斛花；发酵；抗氧化；美白

中图分类号：TS201.3    文献标识码：A    文章编号：1674-506X（2023）06-0072-0005

Study on Antioxidant and Whitening Activities of Alcohol Extracts from Fermented Dendrobium candidum Flowers

JIANG Lin^1，2, HUANG Lisen^1，2, FU Shanshan^1，2, WANG Guihong^1，2, PANG Haiyue^1，2*

（1.Department of Public Health and Medical Technology, Xiamen Medical College, Xiamen Fujian 361023, China;

2.Engineering Research Center of Natural Cosmeceuticals College of Fujian Province, Xiamen Fujian 361023, China）

Abstract：The aim was to explore the effect of microbial fermentation on the functional activity of dendrobium officinale flowers. Pichia farinosa, Saccharomyces cerevisiae and Lactococcus lactis were respectively used for performing pure culture fermentation on the Dendrobium officinale flowers, the ethanol extract of fermented Dendrobium candidum flowers was obtained by ultrasonic extraction with 80% ethanol as the solvent. The contents of flavonoids and total phenols were determined. The DPPH free radical, ABTS^＋ free radical scavenging capacity and total antioxidant capacity were measured to explore the antioxidant activity before and after fermentation and the whitening effect of inhibiting tyrosinase activity. The highest flavonoid content in the ethanol extract of Dendrobium candidum flowers fermented by Pichia pastoris was 13.17 mg/g, and the highest total phenol content in the ethanol extract of Dendrobium candidum flowers fermented by Saccharomyces cerevisiae was 1.04 mg/g. With the increase of extract concentration, the overall antioxidant capacity of the extract was gradually enhanced. Under the condition of 1 mg/mL, the DPPH free radical scavenging rate of ethanol extract from Dendrobium candidum flowers fermented by S. cerevisiae was 81.5%, the highest clearance rate of ABTS^＋ free radical was 66.9% , the strongest total antioxidant capacity was 1.03 mmol/g, and the highest tyrosinase inhibition rate of ethanol extract of Dendrobium candidum flowers fermented by lactic acid bacteria was 37.8%.

Keywords：Dendrobium candidum flower；fermentati；antioxidation；whitening

doi：10.3969/j.issn.1674-506X.2023.06-012

收稿日期：2023-03-16

基金项目：福建省教育厅中青年项目（JAT210485，JAT190850）；天然化妆品福建省高校工程研究中心运行经费

作者简介：江林（2001-），男，本科生。研究方向：天然产物开发。

*通信作者：庞海月（1989-），女，硕士，实验师。研究方向：天然产物开发。

引用格式：江林,黄立森,付姗姗,等. 发酵铁皮石斛花醇提物的抗氧化和美白活性研究[J].食品与发酵科技,2023,59(6):72-76.