李文博，童 颖^*

（武汉药品医疗器械检验所，湖北武汉 430075）

摘要：建立分散固相萃取-高效液相色谱-串联质谱法同时测定豆芽中11种植物生长调节剂和抗生素药物残留的分析方法。样品经过1%甲酸乙腈超声提取，无水硫酸镁和C₁₈净化，用CAPCELL C₁₈色谱柱分离目标物，采用正负切换多反应监测（multiple reaction monitoring，MRM）离子扫描模式对其进行分析。11 种目标化合物在 2.0～100.0 μg/L范围内相关性良好（r≥0.999 3）；在5、10、20 μg/kg 3个添加水平进行加样回收实验，平均回收率范围为80.7%～102.7%，相对标准偏差为1.1%～7.1%（n＝6）；检出限为0.04～0.25 μg/kg，定量限为0.15～0.84 μg/kg。该方法快捷简单、灵敏度好、准确度高，能够满足豆芽中11种植物生长调节剂和抗生素药物残留的检测要求。

关键词：豆芽；植物生长调节剂；抗生素类药物；分散固相萃取；高效液相色谱-串联质谱法

中图分类号：TS207.3    文献标识码：A    文章编号：1674-506X（2022）04-0123-0009

Simultaneous Determination of 11 Plant Growth Regulators and Antibiotic Residues in Bean Sprouts by DSPE-HPLC-MS/MS

LI Wenbo, TONG Ying^*

（Wuhan Institute for Drug and Medical Device Control, Wuhan Hubei 430075, China）

Abstract：To develop a method for the determination of 11 plant growth regulators and antibiotic residues in bean sprouts by the dispersive solid- phase extraction-high performance liquid chromatography-tandem mass spectrometry. The samples were ultrasonically extracted with 1% formic acid in acetonitrile, and then subjected into a purification using C₁₈ and magnesium sulfate (MgSO₄). The target compounds were separated at the CAPCELL C₁₈ column, detected by tandem mass spectrometry in positive and negative ESI modes together with multiple reaction monitoring (MRM). The calibration curves ranging from 2.0 to 100.0 μg/L showed good linearity, while the obtained correlation coefficients were larger than 0.999 3. The average recoveries were 80.7%～102.7% when using spiked levels of 5, 10 and 20 μg/kg, and the relative standard deviations ranged from 1.1% to 7.1% (n＝6). Moreover，the limits of detection (LOD) and limits of quantitation (LOQ) were 0.04～0.25 μg/kg and 0.15～0.84 μg/kg, respectively. This method is simple, sensitive and accurate, and might be suitable for the determination of 11 plant growth regulators and antibiotic residues in bean sprouts.

Keywords：bean sprouts; plant growth regulator; antibiotic; dispersive solid phase extraction; high performance liquid chromatography-tandem mass spectrometry

doi：10.3969/j.issn.1674-506X.2022.04-021

收稿日期：2022-04-09

作者简介：李文博（1989-），男，硕士，主管药师。研究方向：食品质量安全研究与检验检测。

*通信作者：童颖（1989-），女，硕士，主管药师，研究方向：食品、药品质量安全控制。

引用格式：李文博,童颖.DSPE-HPLC-MS/MS法同时测定豆芽中11种植物生长调节剂和抗生素药物残留[J].食品与发酵科技,2022,58(4):123-131.