王丽君，朱书强，李 雁，文书新，周 佳^*

（1.甘肃省食品检验研究院，甘肃兰州 730000；

2甘肃省地理标志产品营养与安全评价工程研究中心，甘肃兰州 730000）

摘要：建立了超高效液相色谱-串联质谱法同时测定含乳饮料等食品中柠檬黄、日落黄、胭脂红、赤藓红、新红、诱惑红、亮蓝、酸性红的检测方法。样品采用氨化乙醇（乙醇∶氨水∶水＝7∶2∶1（v/v/v））提取，纯水洗涤、聚酰胺粉吸附，氨化乙醇再次洗脱。洗脱液在 70 ℃条件下减压旋蒸至干，用水溶解后调pH值至中性。经 Waters ACQUITY UPLC BEH C18（2.1 mm×100 mm，1.7 μm）分离。在流速0.3 mL/min下以乙腈-5 mmol/L乙酸铵溶液作为流动相梯度洗脱。实验采用三重四极杆电喷雾离子源（ESI源）电离-多反应监测（multiple reaction monitoring，MRM）-正离子监测模式检测。8种合成色素分别在20～500 mg/kg 范围内线性关系良好，相关系数R²均大于0.990。加标回收率为79.6%～94.1%，RSD值为4.14%～14.32%。该方法提取率高，分析时间短，准确度高，检出限低，解决了复杂基质给液相色谱分离带来的干扰。

关键词：8种合成色素；含乳饮料；液相色谱-串联质谱法

中图分类号：TS207.3    文献标识码：A    文章编号：1674-506X（2022）04-0132-0005

Simultaneous Determination of 8 Synthetic Pigments in Dairy Beverages by Liquid Chromatography-tandem Mass Spectrometry

WANG Lijun, ZHU Shuqiang, LI Yan, WEN Shuxin, ZHOU Jia^*

（1.Gansu Institute of Food Inspection, Lanzhou Gansu 730000, China;

2.Engineering Research Center of Nutrition and Safety Evaluation of Geographical Indication Products in Gansu Province, Lanzhou Gansu 730000, China）

Abstract：To establish an ultra performance liquid chromatography-tandem mass spectrometry method for simultaneous determination of lemon yellow, sunset yellow, carmine red, erythrum red, new red, lure red, bright blue and acid red in food containing milk drinks. The samples were extracted with ammoniated ethanol (ethanol:ammonia:water, volume ratio:7:2:1), washed with pure water, adsorbed with polyamide powder, eluted with ammoniated ethanol again. The eluent was evaporated to dry at 70 ℃, dissolved in water，and the pH was adjusted to neutral. Through ACQUITY UPLC BEH C18 (2.1 mm×100 mm, 1.7 μm) separation, At the flow rate of 0.3 mL/min, acetonitrile-5 mmol/L ammonium acetate solution was used as mobile phase gradient elution. Triple quadrupole spray ion source (ESI source) ionization- multiple reaction monitoring (MRM) -positive ion monitoring mode was used in the experiment. The linear range of the 8 synthetic pigments was 20～500 mg/kg, and the correlation coefficients R² were all greater than 0.990. The recoveries ranged from 79.6% to 94.1%, and the RSD ranged from 4.14% to 14.32%. This method has the advantages of high extraction rate, short analysis time, high accuracy and low detection limit, which solves the interference caused by complex matrix.

Keywords：8 synthetic pigments; milk drinks; liquid chromatography-tandem mass spectrometry

doi：10.3969/j.issn.1674-506X.2022.04-022

收稿日期：2021-12-21

基金项目：甘肃省市场监督管理局科技计划项目（SSCJG-SP-202114）；知识产权计划-地理标志保护运用类项目（21ZSCQ072）

作者简介：王丽君（1981-），女，本科，工程师。研究方向：食品安全分析与检测。

*通信作者：周佳（1983-），女，硕士，高级工程师。研究方向：食品安全分析监测。

引用格式：王丽君,朱书强,李雁,等.液相色谱-串联质谱法同时测定含乳饮料等食品中8种合成色素[J].食品与发酵科技,2022,58(4):132-136.