胡 杨1，李先芝1，2*，严 玲1，毛琼丽1，刘 洋1，石 豪1
（1.劲牌有限公司，湖北大冶 435112；
2.中药保健食品质量与安全湖北省重点实验室，湖北大冶 435112）
摘要：建立HPLC法同时测定赶黄草药材及醇提物中没食子酸、儿茶素、槲皮苷、槲皮素的含量，为赶黄草药材及醇提物的质量评价提供参考。以60%甲醇作为提取溶剂，超声提取30 min。采用YMC色谱柱（4.6 mm×250 mm，5 μm），乙腈-0.1%磷酸作为流动相梯度洗脱，流速1.0 mL/min，柱温30 ℃，检测波长205 nm；进样量10 μL。结果表明，4 种化合物成分在各自范围内线性关系良好（r≥0.999 9），平均加标回收率 96.15%～100.21%，RSD 值在0.74%～1.79%之间。实验结果表明该方法操作简单，准确度高，重复性好，可用于赶黄草药材及醇提物样品中的含量测定及产品质量监控。
关键词：赶黄草；醇提物；含量测定
中图分类号：TS207.3 文献标识码：A 文章编号：1674-506X（2022）05-0118-0007
Simultaneous Determination of the Contents of 4 Active Components in Penthorum chinense and Its Alcohol Extracts by HPLC
HU Yang1, LI Xianzhi1，2*, YAN Ling1, MAO Qiongli1, LIU Yang1, SHI Hao1
（1.Jing Brand Co., Ltd., Daye Hubei 435112, China;
2.Hubei Provincial Key Lab for Quality and Safety of Traditional Chinese Medicine Health Food, Daye Hubei 435112, China）
Abstract：To establish an HPLC method to simultaneously determine the contents of gallic acid, catechin，quercetin, and quercetin in the materials and alcohol extracts of Penthorum chinense, and provide reference for the quality evaluation of materials and alcohol extracts of Penthorum chinense. With 60% methanol as the extraction solvent, ultrasonic extraction was performed for 30 min. Use YMC column (4.6 mm×250 mm, 5 μm) Acetonitrile 0.1% phosphoric acid was used as the mobile phase for gradient elution, the flow rate was 1.0 mL/min, the column temperature was 30 ℃, and the detection wavelength was 205 nm; Injection volume 10 μL. The four compounds have a good linear relationship within their respective ranges (r≥0.999 9), the average recovery rate of standard addition is 96.15%～100.21%, and the RSD value is between 0.74%～1.79%. The experimental results show that the method is simple to operate, accurate, and reproducible. It can be used for the content determination and product quality monitoring of Penthorum chinense materials and alcohol extract samples.
Keywords：Penthorum chinense Pursh; alcohol extract; content determination
doi：10.3969/j.issn.1674-506X.2022.05-020
收稿日期：2022-01-10
基金项目：湖北省科技计划项目（2018ACA125）
作者简介：胡杨（1993-），男，硕士，工程师。研究方向：产品质量控制。
*通信作者：李先芝（1978-），女（土家族），硕士，高级工程师。研究方向：产品质量控制。
引用格式：胡杨,李先芝,严玲,等.HPLC 法同时测定赶黄草药材及其醇提物中4种有效成分含量[J].食品与发酵科技,2022,58(5):118-123,136.
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