聂增宇，胡文林，尚学平，谢凤娇，许世锦，陈罗华周

（广东天益生物科技有限公司，广东湛江 524300）

摘要：以紫色红曲菌为出发菌株，在200 L发酵罐中进行液态深层发酵生产莫纳可林K（简称MK）。为得到含量最高的MK，在发酵培养基不变的条件下，分别以罐压、通气量和搅拌转速为因素进行单因素试验，确定因素水平后，以MK为评价指标进行Box-Behnken响应面优化，确定该菌株产MK的最佳中试发酵工艺：罐压为0.06 MPa，通气量为22 m³/h，搅拌转速为134 r/min。结果表明，在此条件下，发酵液中MK含量为828 mg/L。本研究获得红曲菌液态深层发酵产MK的最佳中试发酵工艺，为大生产及后续研究提供理论依据。

关键词：红曲菌；莫纳可林K；液态发酵；响应面法

中图分类号：TQ920.6   文献标识码：A   文章编号：1674-506X（2022）06-0072-0006

Optimization of Monacolin K Production by Liquid Fermentation of Monascus Using Response Surface Methodology

NIE Zengyu, HU Wenlin, SHANG Xueping, XIE Fengjiao, XU Shijin, CHENLUO Huazhou

（Guangdong Tianyi Biotechnology Co., Ltd., Zhanjiang Guangdong 524300, China）

Abstract：Monascus purpureus was used as the starting strain to produce Monacolin K (MK) by liquid fermentation in 200 L fermenter. In order to obtain the highest MK, the single-factor experiment was carried out with the tank pressure, the aeration rate and the agitation speed as the factors under the condition of constant fermentation medium, the optimization of Box-Behnken response surface was carried out with MK as the evaluation index, and the optimal medium-scale fermentation process was determined as follows: tank pressure 0.06 MPa, aeration 22 m³/h, agitation speed 134 r/min. The results showed that the content of MK in the fermentation broth was 828 mg/L. In this study, the optimum medium- scale fermentation process of Monascus MK production by liquid fermentation was obtained, which provided theoretical basis for large-scale production and follow-up research.

Keywords：Monascus; Monacolin K; liquid fermentation; response surface methodology

doi：10.3969/j.issn.1674-506X.2022.06-011

收稿日期：2022-09-08

基金项目：湛江市科技计划项目（2020A05002）

作者简介：聂增宇（1986-），男，本科，助理工程师。研究方向：红曲发酵产品的生产及研究。

引用格式：聂增宇,胡文林,尚学平,等.响应面法优化红曲菌液态发酵产莫纳可林K工艺[J].食品与发酵科技,2022,58(6):72-76,82.