刘秀娟，曹 秀，余明霞，董小海，郝学飞^*

（河南省农业科学院农业质量标准与检测技术研究所，河南郑州 450002）

摘要：通过对样品净化方式和仪器条件的优化，建立动物源性食品中利巴韦林的检测方法。样品经水溶液提取，经过HyperSep HyperCarb固相萃取柱净化，采用同位素内标法定量，经超高效液相色谱-串联质谱联用仪进行检测。结果表明，利巴韦林在0.50～10.0 μg/L 范围内线性关系良好，相关系数大于0.999，样品加标平均回收率在89.9%～102.7%之间，相对标准偏差为3.84%～8.09%。该方法净化效果好，回收率稳定、精密度良好，灵敏度高，适用于动物源性食品中利巴韦林的定量分析。

关键词：动物源性食品；固相萃取；利巴韦林；净化；超高效液相色谱-串联质谱法

中图分类号：TS207.3    文献标识码：A    文章编号：1674-506X（2022）06-0127-0005

Determination of Ribavirin in Animal Derived Food by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

LIU Xiujuan, CAO Xiu, YU Mingxia, DONG Xiaohai, HAO Xuefei^*

（Institute of Agricultural Quality Standards and Testing Technology, Henan Academy of Agricultural Sciences, Zhengzhou Henan 450002, China）

Abstract：Via the selection and optimization of extraction solvents and apparatus parameters, an efficient method for the determination of ribavirin in animal derived food was developed. Samples were extracted by water, purified by solid phase extraction with HyperSep HyperCarb, quantified using isotope internal standard and detected by ultra high performance liquid chromatography-tandem mass spectrometry. The results show that ribavirin had good linearities in the range of 0.50～10.0 μg/L, with the correlation coefficient more than 0.999. The recoveries were from 89.9% to 102.7%, and the relative standard deviations were from 3.84% to 8.09%. This method has good purification, recovery rate, precision and sensitive, and it is suitable for quantitative analysis of ribavirin in animal derived food.

Keywords：animal derived food; solid phase extraction; ribavirin; purification; ultra high performance liquid chromatography-tandem mass spectrometry

doi：10.3969/j.issn.1674-506X.2022.06-020

收稿日期：2022-01-10

作者简介：刘秀娟（1981-），女，硕士，工程师。研究方向：农产品质量安全检测。

*通信作者：郝学飞（1979-），男，硕士，副研究员。研究方向：农产品质量安全。

引用格式：刘秀娟,曹秀,余明霞,等.超高效液相色谱—串联质谱法测定动物源性食品中利巴韦林[J].食品与发酵科技,2022,58(6):127-131.