程春梅
(浙江药科职业大学,浙江宁波 315500)
摘要:建立了利用高效液相色谱测定食用植物油中的呕吐毒素的方法。实验采用真菌毒素多功能净化柱对食用植物油中呕吐毒素提取液进行净化,用C18色谱柱(250 mm×4.6 mm,5 μm),以甲醇-水溶液(20∶80,v/v)为流动相,流速1.0 mL/min,柱温25 ℃,二极管阵列检测器在波长218 nm处进行检测。被测样品加标回收率为93.6%~99.4%,RSD为4.33%~11.39%。
关键词:食用植物油;呕吐毒素;高效液相色谱法;真菌毒素多功能净化柱
中图分类号:TS207.3 文献标识码:A 文章编号:1674-506X(2022)06-0132-0004
Determination of Vomitoxin in Edible Vegetable Oil by High Performance Liquid Chromatography
CHENG Chunmei
(Zhejiang Pharmaceutical University, Ningbo Zhejiang 315500, China)
Abstract:A HPLC method is proposed for the determination of vomitoxin in edible vegetable oil. The multifunctional purification column of mycotoxin was used to purify the extract of vomittin in edible vegetable oil. The separation was achieved by using a C18 column (250 mm×4.6 mm, 5 μm) with methanol-water (20∶80, v/v) as eluent, flow rate 1.0 mL/min, column temperature 25 ℃, lastly detected at 218 nm through a photo-diode array detector. With this method, good linear relationship, sensitivity and reproducibility were obtained. Rate of recovery ranged from 93.6% to 99.4%, RSD ranged from 4.33% to 11.39%.
Keywords:HPLC; vomitoxin; edible vegetable oil; mycotoxin multifunctional clean-up column
doi:10.3969/j.issn.1674-506X.2022.06-021
收稿日期:2022-03-21
基金项目:浙江省教育厅 2018 年度高校访问工程师“校企合作项目”(FG2018007);宁波市公益类科技计划项目(202002N3088)
作者简介:程春梅(1979-),女,硕士,教授。研究方向:食品贮运保鲜、食品质量安全检测。
引用格式:程春梅.液相色谱法测定食用植物油中的呕吐毒素含量[J].食品与发酵科技,2022,58(6):132-134,140.
全文查看