房翠兰²，王贵敏²，向 利²，肖 伦²，冉秀芝^1*

（1.重庆理工大学化学化工学院，重庆 400054；

2.重庆市九龙坡区疾病预防控制中心，重庆 400039）

摘要：以市售常见的水产品为研究对象，建立并优化了QuEChERS结合LC-MS-MS测定水产品中8种多环芳烃残留量的方法。样品经超声辅助乙腈提取，经C18和弗洛里硅土双连接层析柱净化后，采用液相色谱-质谱检测器对多环芳烃进行检测分析。结果表明，8种多环芳烃分离效果好，检出限（LOD）为0.006～0.105 μg/kg，相对标准偏差为0.3%～4.0%（n＝4），加标回收率达到86%～110%。这表明该方法具有比较高的重现性和选择性，对于水产品中多环芳烃的残留测定具有很好的应用价值。

关键词：QuEChERS；多环芳烃；液相色谱-质谱；水产品

中图分类号：TS207.3   文献标识码：A   文章编号：1674-506X（2021）03-0152-0006

Determination of 8 Polycyclic Aromatic Hydrocarbons in Aquatic Products by LC-MS-MS Coupled with Optimized QuEChERS

FANG Cui-lan²，WANG Gui-min²，XIANG Li²，XIAO Lun²，RAN Xiu-zhi^1*

（1.College of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China;

2.Chongqing Jiulongpo District Center for Disease Control and Prevention, Chongqing 400039, China）

Abstract：By taking the common aquatic products on the market as material，an analytical method for the determination of 8 polycyclic aromatic hydrocarbons (PAHs) in aquatic products by liquid chromatography-mass spectrometry was established. The sample was extracted by ultrasonic assisted acetonitrile, and the extract was cleaned up with C18 and Florisil chromatographic bi-column, detected with LC-MS/MS for PAHs. The results showed that the 8 PAHs could be separated efficiently. The detection limits of this method were 0.006～0.105μg/kg. The relative standard deviations were ranging from 0.3%～4.0% (n＝4), and the recovery rates were from 86%～110%. The method proposed by this paper possesses higher producibility and selectivity, and has promising applications in determining the PAHs in aquatic products．

Keywords：QuEChERS; polycyclic aromatic hydrocarbons; LC-MS-MS; aquatic products

doi:10.3969/j.issn.1674-506X.2021.03-024

收稿日期：2020-10-11

基金项目：重庆市九龙坡区科技局社会事业类项目（项目编号：社201808）

作者简介：房翠兰（1976-），女，硕士研究生，工程师。研究方向：理化检测。

*通信作者

引用格式：房翠兰,王贵敏,向利,等.优化QuEChERS结合LC-MS-MS法测定水产品中8种多环芳烃的研究[J].食品与发酵科技,2021,57(3):152-156,163.