李南臻^1，2，曾志刚^1*，王 刚²，万玉军²，陈沿言¹

（1.抗生素研究与再评价四川省重点实验室，四川抗菌素工业研究所，药学院，成都大学，四川成都 610106；

2.四川省食品发酵工业研究设计院有限公司，四川成都 611130）

摘要：为了快速测定废菌丝体中麦角甾醇的含量，本文对紫外分光光度法进行了考察。以二氯甲烷为提取溶剂，麦角甾醇在0.005～0.025 mg/mL范围内线性关系良好，相关系数为0.999 79，平均回收率为98.87%～103.65%，RSD值为2.03%～3.07%，样品在10 h内稳定。本方法具有设备操作简便、检测快速的特点，适用于即时检测，在安全性、精密度和稳定性方面良好，可用于废菌丝体中麦角甾醇的快速测定。

关键词：米曲霉；废菌丝体；麦角甾醇；紫外分光光度法

中图分类号：TS207.3   文献标识码：A   文章编号：1674-506X（2021）03-0157-0004

Rapid Determination of Ergosterol in Waste Mycelium by UV Spectrophotometry

LI Nan-zhen^1，2, ZENG Zhi-gang^1*, WANG Gang², WAN Yu-jun², CHEN Yan-yan¹

（1.Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, Chengdu Sichuan 610106, China;

2.Sichuan Food and Fermentation Industry Research & Design Institute Co., Ltd., Chengdu Sichuan 611130, China）

Abstract：In order to quickly determine the content of ergosterol in waste mycelium, this paper examined the ultraviolet spectrophotometry. The extraction solvent was dichloromethane. The calibration curve was linear over the concentration range from 0.005 to 0.025 mg/mL, with a correlation coefficient of 0.999 79. The average recovery was 98.87%～103.65%, and the relative standard deviation (RSD) range was 2.03%～3.07% . The sample was stable within 10 hours. This method has the characteristics of simple equipment operation and rapid detection, which are suitable for immediate detection. The method has good safety, precision and stability, and can be used for the rapid determination of ergosterol in waste mycelium.

Keywords：Aspergillus oryzae; waste mycelium; ergosterol; uv spectrophotometry

doi：10.3969/j.issn.1674-506X.2021.03-025

收稿日期：2021-03-08

基金项目：酶资源四川省科技资源共享服务平台（2020JDPT0018）

作者简介：李南臻（1990-），男，硕士研究生，工程师。研究方向：微生物与生化药学。

*通信作者

引用格式：李南臻,曾志刚,王刚,等.紫外分光光度法快速测定废菌丝体中麦角甾醇的含量[J].食品与发酵科技,2021,57(3):157-160.