林文杰¹，杨 婧¹，胡湘婷¹，周 明²，赵治巧¹，秦依涵¹，陈 惠¹，布同良¹，唐自钟^1*，周文川²

（1.四川农业大学生命科学学院，四川雅安 625014；

2.大竹县万康生态农业发展有限公司，四川达州 635100）

摘要：本研究对1株大竹地区采集的蝉花进行分离和鉴定，分析比较其子实体提取物（ZST）与发酵菌丝体提取物（JST）的抗氧化活性。结果表明，供试菌株为虫草属（Cordyceps）金蝉花（大蝉草）（Cordyceps cicadae）；ZST的多糖、蛋白质和多酚含量分别为JST的1.51、1.16和1.85倍。ZST对1，1-二苯基-2-三硝基苯肼（DPPH）自由基、羟基（·OH）自由基、2，2-联氮-二（3-乙基—苯并噻唑-6-磺酸）二铵盐（ABTS）自由基和超氧阴离子（·O₂^-）自由基的IC₅₀值分别为0.65±0.002 mg/mL、0.42±0.007 mg/mL、0.26±0.020 mg/mL和1.64±0.058 mg/mL。而JST对DPPH、·OH、ABTS和·O₂^-自由基的清除能力的IC₅₀值分别为1.33±0.096mg/mL、0.70±0.014mg/mL、0.76±0.031mg/mL和2.35±0.399mg/mL，即ZST抗氧化性强于JST，可能由于其含有更多的多糖、蛋白质和多酚等抗氧化活性物质。该研究可以为大竹地区蝉花资源的开发提供重要的参考价值。

关键词：金蝉花；分离与鉴定；提取物；抗氧化

中图分类号：R284.2；R285.5   文献标识码：A   文章编号：1674-506X（2021）05-0022-0007

Isolation and Identification of Wild Cordyceps cicadae and Antioxidant Activities of Extract

LIN Wen-jie¹, YANG Jing¹, HU Xiang-ting¹, ZHOU Ming², ZHAO Zhi-qiao¹, QIN Yi-han¹, CHEN Hui¹, BU Tong-liang¹, TANG Zi-zhong^1*, ZHOU Wen-chuan²

（1.College of Life Science, Sichuan Agricultural University, Ya’an Sichuan 625014, China;

2.Dazhu Wankang Ecological Agriculture Development Co., Ltd., Dazhou Sichuan 635100, China）

Abstract：In this study, a wild Cordyceps cicadae strain in Dazhu were isolated and identified, and the antioxidant activities of extract (ZST and JST) from the fruiting body and the fermentation mycelium were analyzed. The results showed that the tested strain was Cordyceps cicadae. The contents of polysaccharider, protein and polyphenols in ZST were 1.51, 1.16 and 1.85 times of those in JST, respectively. The IC₅₀ values of ZST for 1, 1-diphenyl-2- picrylhydrazyl (DPPH) radical, hydroxyl (·OH) radical, 2, 2′-Azinobis -(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical and superoxide anion (·O₂^-) radical were 0.65±0.002 mg/mL, 0.42±0.007 mg/mL, 0.26±0.020 mg/mL and 1.64±0.058 mg/mL, respectively. The IC₅₀ values of DPPH, ·OH, ABTS and · O₂^-free radicals of JST were 1.33±0.096 mg/mL, 0.70±0.014 mg/mL, 0.76±0.031 mg/mL and 2.35±0.399 mg/mL, respectively. In other words, the antioxidant activity of ZST was greater than that of JST, which may be related to the higher polysaccharides, proteins and polyphenols content. This study can provide important reference value for the development of Cordyceps cicadae resources in Dazhu.

Keywords：Cordyceps cicadae；isolation and identification；extract；antioxidation

doi：10.3969/j.issn.1674-506X.2021.05-004

收稿日期：2021-08-18

基金项目：本研究由金蝉花多糖饮品研究项目资助（2022239002）。

作者简介：林文杰（1996-），女，硕士研究生。研究方向：应用生物化学。

*通信作者

引文格式：林文杰,杨婧,胡湘婷,等.野生蝉花的分离鉴定及提取物抗氧化活性研究[J].食品与发酵科技,2021,57(5):22-27,35.