罗丽娟¹，王 刚^1*，万玉军¹，关统伟², 李南臻¹，庹有朋¹，吴 泽¹，苟红梅¹

（1.四川省食品发酵工业研究设计院有限公司生物发酵研究所，四川成都 611130；

2.西华大学食品与生物工程学院，四川成都 610039）

摘要：本研究从实验室保藏的产γ-聚谷氨酸地衣芽孢杆菌作为出发菌株，采用⁶⁰Co-γ射线辐照和ARTP诱变协同复合诱变技术，筛选出了一株γ-聚谷氨酸高产菌株FA-22，产量达20.63 g/L。通过对其培养基组分和摇瓶培养条件优化，确定了菌株FA-22的最适发酵培养基组分：L-谷氨酸20.0 g/L、柠檬酸12.0 g/L、甘油90.0 g/L、NH₄Cl 7.0 g/L、K₂HPO₄ 0.5 g/L、MgSO₄·7H₂O 0.5 g/L、FeCl₃·6H₂O 0.04 g/L、CaCl₂·6H₂O 0.15 g/L、MnSO₄·H₂O 0.104 g/L，pH值7.5；最佳摇瓶培养条件：接种量10%、转速220 r/min、温度37 ℃，装液量80 mL/500 mL、发酵周期72 h；在此条件下，γ-聚谷氨酸产量达32.53 g/L，较优化前产量提升了57.68%，为后期的中试发酵奠定了良好的基础。

关键词：γ-聚谷氨酸；诱变；发酵；优化

中图分类号：TQ920.1   文献标识码：A   文章编号：1674-506X（2021）06-0035-0008

Breeding of High-yield γ-polyglutamic Acid Strains and Optimization of Fermentation Conditions

LUO Li-juan¹, WANG Gang^1*, WAN Yu-jun¹, GUAN Tong-wei², LI Nan-zhen¹, TUO Yuo-peng¹, WU Ze¹, GOU Hong-mei¹

（1.Institute of Biological Fermentation, Sichuan Food and Fermentation Industry Research & Design Institute Co., Ltd.,

Chengdu Sichuan 611130, China;

2.College of Food and Biological Engineering, Xihua University, Chengdu Sichuan 610039, China）

Abstract：In this study, the γ-polyglutamic acid-producing Bacillus licheniformis in the laboratory was used as the starting strain, using ⁶⁰Co-γ ray irradiation and ARTP mutagenesis combined mutagenesis technology to screen a high-yielding γ-polyglutamic acid strain FA-22, with a yield of 20.63 g/L. By optimizing its medium components and shaking flask culture conditions, the optimum fermentation medium components of strain FA-22 were determined: L-glutamic acid 20.0 g/L, citric acid 12.0 g/L, glycerol 90.0 g/L, NH₄Cl 7.0 g/L, K₂HPO₄ 0.5 g/L, MgSO₄·7H₂O 0.5 g/L, FeCl₃·6H₂O 0.04 g/L, CaCl₂·6H₂O 0.15 g/L, MnSO₄·H₂O 0.104 g/L, pH 7.5; The optimal shaking flask culture conditions were as follows: inoculation amount of 10%, rotation speed of 220 r/min, temperature of 37 ℃, liquid volume of 80 mL/500 mL, fermentation period of 72 h; Under these conditions, the yield of γ- polyglutamic acid was up to 32.53 g/L, 57.68% higher than that before optimization, which laid a good foundation for the later pilot fermentation.

Keywords：γ-polyglutamic acid; mutagenesis; fermentation; optimization

doi：10.3969/j.issn.1674-506X.2021.06-006

收稿日期：2021-11-10

基金项目：四川省教育厅对口科技援助项目（2021YFQ0016）；酶资源四川省科技资源共享服务平台（2020JDPT0018）

作者简介：罗丽娟（1992-），女，硕士，工程师。研究方向：微生物发酵。

*通信作者

引文格式：罗丽娟,王刚,万玉军,等.γ-聚谷氨酸高产菌株选育及发酵条件优化[J].食品与发酵科技,2021,57(6):35-42.