闫舒雅，郭素萍^*

（山西省药品检查中心（山西省疫苗检查中心），山西太原 030006）

摘要：本研究以桦褐孔菌为研究对象，通过响应面法对桦褐孔菌发酵产多糖条件进行优化，以多糖量为指标。通过单因素及Box-Bchnken响应面优化，确定该菌丝发酵产多糖最佳条件：pH为8，接种量为装液量的12.5%，发酵时间为12 d。结果表明，在此条件下多糖量为20.547 g/L。本研究获得了桦褐孔菌产多糖最佳发酵条件，为多糖的大量制备及其他后续研究提供理论依据。

关键词：桦褐孔菌；多糖；响应面法

中图分类号：TQ929   文献标识码：A   文章编号：1674-506X（2021）06-0049-0005

Optimization of Fermentation Process of Inonotus obliquus Polysaccharides by Response Surface Methodology

YAN Shu-ya, GUO Su-ping^*

（Shanxi Provincial Drug Inspection Center （Shanxi Provincial Vaccine Inspection Center）, Taiyuan Shanxi 030006, China）

Abstract：Using Inonotus obliquus as the research object in this study, and the conditions for the fermentation of polysaccharides from Inonotus obliquus were optimized by response surface methodology. Using polysaccharide as an indicator，the bacteria was determined by single factor and Box-Bchnken response surface optimization. The best conditions of polysaccharide production by mycelium fermentation: pH 8, inoculation volume 12.5% of the liquid volume and fermented 12 days. The results show that under this condition, the amount of polysaccharide is 20.547 g/L. This study obtained the best fermentation conditions for the production of polysaccharides from Inonotus obliquus, providing a theoretical basis for the large-scale preparation of polysaccharides and other follow-up studies.

Keywords：Inonotus obliquus; polysaccharides; response surface methodology

doi：10.3969/j.issn.1674-506X.2021.06-008

收稿日期：2020-06-02

基金项目：科技部科技援助项目（KY20110097）

作者简介：闫舒雅（1987-），女，硕士，助理工程师。研究方向：微生物生理特性的研究。

*通信作者

引文格式：闫舒雅,郭素萍.响应面法优化桦褐孔菌发酵产多糖工艺[J].食品与发酵科技,2021,57(6):49-53.